Accelerate to Discover

Back to filter

Related topics

Introducing IncuCyte antibody Internalization assays

Essen Bioscience

Jan 22, 2018

An integrated solution to automatically visualize and quantify antibody internalization, in real time and in 96-well...

Long-read sequencing of coffee bean transcriptome reveals the diversity of full-length transcripts

Sage Science

Jan 19, 2018

In this study, researchers generated the transcriptome of the tetraploid Arabica coffee bean, focusing on caffeine and...

Studying cancer biology and therapeutic targets using PET technology

Bruker Biospin

Jan 18, 2018

In vivo imaging is at the heart of preclinical research into cancer biology and treatment. In this webinar, you will...

High-frequency ultrasound imaging of mouse cervical lymph nodes

VisualSonics FujiFilm

Dec 7, 2017

The video describes the protocol of application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph...

Jan 22, 2018

Optical tweezers measurements of DNA conformational dynamics at the single-molecule level

LUMICKS

Dec 4, 2017

The real-time observation of DNA breathing shows the ability of LUMICKS’ C-Trap to investigate DNA breathing...

Launching Bionano U

BioNano Genomics

Nov 30, 2017

Discover what is missing in your gene mapping research with new Bionano U. This Bionano web product is a dynamic...

Stem cell differentiation is tuned by oxygen concentration and pressure

Xcell Biosciences

Nov 29, 2017

Stem cells have vast potential for use in regenerative medicine due to their ability to differentiate into multiple...

Jan 22, 2018

Albira Si enables enhanced imaging in cancer research

Bruker Biospin

Nov 28, 2017

More than 1.5 million people are estimated to receive a cancer diagnosis in 2017 in the United States. Imaging of HER3...

Show all topics (10)

Automated continuous monitoring of growth factor-mediated Endothelial cell migration

Dec 5, 2017

The integrated nature of cell migration is exemplified by angiogenesis. Angiogenesis or neo-angiogenesis refers to the formation of new blood vessels from pre-existing vessels and is critical for development, wound healing and tumor growth. Endothelial cell migration is an important component of angiogenesis, involving chemotactic, haptotactic and mechanotactic (shear stress) induced cell migration. Chemotactic cell migration is typically induced by soluble growth factors such as vascular endothelial growth factor (VEGF) and its isoforms, fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) amongst others. These growth factors interact with their cognate receptor tyrosine kinases on he surface of endothelial cells activating signaling pathways culminating in directed cell migration.

In the present study, we used the CIM-Plate 16 with the xCELLigence RTCA DP Instrument to monitor growth factor-mediated migration of endothelial cells in realtime using label-free conditions. The CIM-Plate 16 is a 16-well modified Boyden chamber composed of an upper chamber (UC) and a lower chamber (LC). The UC and LC easily snap together to form a tight seal. The UC is sealed at its bottom by a microporous Polyethylene terephthalate (PET) mem-brane. These micropores permit the physical translocation of cells from the upper part of the UC to the bottom side of the membrane. The bottom side of the membrane (the side facing the LC) contains interdigitated gold microelectrode sensors which will come in contact with migrated cells and generate an impedance signal. The LC contains 16 wells, each of which serves as a reservoir for a chemoattractant solution on the bottom side of the wells, separated from each other by pressure-sensitive O-ring seals.

Read more

Scientific paper
Application

Related technologies: Real-time, Label Free Cell Analysis

Get more info

Riccardo Pasculli

Field Application Specialist

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey