Accelerate to Discover

Back to filter

Related topics

Inhibition of T cell receptor signaling mediated Calcium flux in T cells

Apr 23, 2018

Calcium (Ca2+) is an important secondary messenger and a key component of many cell processes. It is an extremely...

High-resolution and high-sensitivity PET imaging of cardiovascular disease

Bruker Biospin

Apr 20, 2018

In this paper, Bruker report the recent progress in preclinical PET hardware development enabling now to reach...

Rapid, accurate diagnosis of Clostridium difficile infections

BJS Biotechnologies

Apr 19, 2018

Real-time PCR of toxin genes has been shown to be the most effective diagnostic method for accurate and early diagnosis...

3D culture systems for 3D world

Synthecon

Apr 18, 2018

Synthecon, Incorporated is a biotechnology company that specializes in the design and manufacture of 3D cell culture...

Apr 24, 2018

Target large genomic regions with 10x genomics using SageHLS

Sage Science

Apr 16, 2018

With customized Cas9 nucleases, scientists can now isolate specific large genomic DNA fragments with the SageHLS...

Expert-led didactic versus self-directed audiovisual training of confocal laser endomicroscopy

Mauna Kea Technologies

Apr 16, 2018

This randomized controlled study involved trainee analysts who were taught how to recognize mucosal barrier defects on...

The effects of hypoxia and hydrostatic pressure in translational research

Xcell Biosciences

Apr 16, 2018

In vitro growth of cells in conditions close to native conditions and comparison to regular in vitro cultivation helped...

Apr 24, 2018

Evaluation of quantum dot toxicity on three-dimensional spheroid using Lumascope720

Etaluma

Apr 12, 2018

In this work, three-dimensional (3D) spheroid cultures of human adipose-derived mesenchymal stem cells (hAD-MSCs), with...

Show all topics (10)

Confocal laser endomicroscopy in head and neck malignancies

Mar 8, 2017

Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients outcome. The feasibility of the tumor antigens epidermal growth factor receptor and epithelial cell adhesion molecule for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas, and represent prognostic and therapeutic tumor target molecules. FITC-conjugated EGF-R- and EpCAM-specific antibodies served as molecular tools for the detection of antigen-positive cells using the CLE technology. Specificity of both antibodies and their ability to discriminate tumor from non-tumor cells were assessed in vitro with human fibroblasts and PCI-1 HNSCC cell lines, and ex vivo on primary HNSCC samples and healthy mucosa. Antigen specificity of the used EpCAM-specific antibody was superior to that of the EGF-R-specific antibody both in vitro and ex vivo, and allowed visualization of cellular structures in CLE measurements. These results hold promise for possible future applications in humans.

Read more

Scientific paper
Application

Related technologies: Probe based Confocal Microscopy

Get more info

Miroslav Vecheta

Support Specialist

Miroslav

Vecheta

+420 737 202 127

Send Message

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey