Accelerate to Discover

Back to filter

Related topics

A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Acea Biosciences

Feb 19, 2018

To increase the immunosurveillance in HIV infection, we used retroviral vectors expressing CD4-chimeric antigen...

BioNano mapping outperforms existing clinical tests in Duchenne´s muscular dystrophy patients

BioNano Genomics

Feb 15, 2018

In a publication in Genome Medicine, a team of researchers from Children’s National Health Center and University of...

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Sartorius

Feb 12, 2018

A  growing  body  of  evidence  suggests  that  more  relevant  and  translational  observations  can  be  made  with ...

Measuring absolute blood perfusion in mice using dynamic contrast-enhanced ultrasound

VisualSonics FujiFilm

Feb 8, 2018

Contrast-enhanced ultrasound imaging involves the injection of gas-filled microbubbles that do not extravasate. This...

Feb 20, 2018

Nanoparticle-uptake behavior in two standard cell lines, NIH/3T3 and A549

Etaluma

Feb 7, 2018

The uptake of nanomaterials into different cell types is a key issue for the determination of nanotoxicity as well as...

Mapping and phasing of structural variation in patient genomes using nanopore sequencing

Sage Science

Feb 6, 2018

Scientists used the Oxford MinION nanopore sequencer with a novel pipeline, NanoSV, to detect structural variants from...

De novo design of a biologically active amyloid

Mauna Kea Technologies

Feb 5, 2018

Short amyloidogenic protein fragment can induce the aggregation of a protein normally not associated with amyloidosis...

Feb 20, 2018

Exploration of melanoma metastases in mice brains using endogenous contrast photoacoustic imaging

VisualSonics FujiFilm

Feb 2, 2018

This recent article by Lavaud et al., uses high-frequency ultrasound and spectroscopic photoacoustic imaging as a...

Show all topics (10)

Exome and genome sequencing of nasopharynx cancer identifies NF-kB pathway activating mutations

Mar 31, 2017

Nasopharyngeal carcinoma (NPC) is an aggressive head and neck cancer characterized by Epstein-Barr virus (EBV) infection and dense lymphocyte infiltration. The scarcity of NPC genomic data hinders the understanding of NPC biology, disease progression and rational therapy design. Here we performed whole-exome sequencing (WES) on 111 micro-dissected EBV-positive NPCs, with 15 cases subjected to further whole-genome sequencing (WGS), to determine its mutational landscape. We identified enrichment for genomic aberrations of multiple negative regulators of the NF-kB pathway, including CYLD, TRAF3, NFKBIA and NLRC5, in a total of 41% of cases. Functional analysis confirmed inactivating CYLD mutations as drivers for NPC cell growth. The EBV oncoprotein latent membrane protein 1 (LMP1) functions to constitutively activate NF-kB signalling, and we observed mutual exclusivity among tumours with somatic NF-kB pathway aberrations and LMP1-overexpression, suggesting that NF-kB activation is selected for by both somatic and viral events during NPC pathogenesis.

Read more

Scientific paper
Application

Related technologies: Target Enrichment for NGS

Get more info

Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

Related products

Superior library preparation on the widest scale with a streamlined workflow and low costs.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey