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How to build the largest genome ever, in 3 easy steps

BioNano Genomics

Feb 23, 2018

Here we report the sequencing and assembly of the 32-gigabase-pair axolotl genome using an approach that combined...

Timelapse imaging of Drosophila melanogaster larva's central nervous system

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Drosophila melanogaster is a well-established model organism to understand biological processes and study human...

High-throughput observation of molecular motor activity & dynamics

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With LUMICKS’ C-Trap, it is possible to observe myosin activity,the processive motion, the binding kinetics along...

A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

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To increase the immunosurveillance in HIV infection, we used retroviral vectors expressing CD4-chimeric antigen...

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BioNano mapping outperforms existing clinical tests in Duchenne´s muscular dystrophy patients

BioNano Genomics

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In a publication in Genome Medicine, a team of researchers from Children’s National Health Center and University of...

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Sartorius

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A  growing  body  of  evidence  suggests  that  more  relevant  and  translational  observations  can  be  made  with ...

Measuring absolute blood perfusion in mice using dynamic contrast-enhanced ultrasound

VisualSonics FujiFilm

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Contrast-enhanced ultrasound imaging involves the injection of gas-filled microbubbles that do not extravasate. This...

Feb 25, 2018

Nanoparticle-uptake behavior in two standard cell lines, NIH/3T3 and A549

Etaluma

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The uptake of nanomaterials into different cell types is a key issue for the determination of nanotoxicity as well as...

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Identification and measurement of bacterial size using the ImageStream

Apr 27, 2016

In this note, we describe two bacterial studies performed using the ImageStream high speed imaging cytometer. In the first system, pathogenic bacteria are distinguished from microflora and debris normally present in alfalfa seed sprouts. Because the aerobic conditions necessary for sprout growth also foster microbial growth, sprouts harbor a diverse microflora, including potential human pathogens. Salmonella spp., probed using a novel FISH method, are detected within complex sprout preparations by combining fluorescence/SSC intensity-based measurements with morphologic confirmation of gated populations. In the second study we measure length of individual bacteria for large populations of E.Coli and Cyanobacteria to follow bacterial growth in culture. By combining high speed image collection with quantitative imagebased measurements, the ImageStream enables accurate identification and classification of bacteria within heterogeneous samples.

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Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

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