Accelerate to Discover

Back to filter

Related topics

A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Acea Biosciences

Feb 19, 2018

To increase the immunosurveillance in HIV infection, we used retroviral vectors expressing CD4-chimeric antigen...

xxcloud – thermal cycler software

BJS Biotechnologies

Feb 16, 2018

Tired of waiting in line to use a qPCR system? The wait is over… the xxcloud is here! The xxcloud is exciting new...

BioNano mapping outperforms existing clinical tests in Duchenne´s muscular dystrophy patients

BioNano Genomics

Feb 15, 2018

In a publication in Genome Medicine, a team of researchers from Children’s National Health Center and University of...

Measuring absolute blood perfusion in mice using dynamic contrast-enhanced ultrasound

VisualSonics FujiFilm

Feb 8, 2018

Contrast-enhanced ultrasound imaging involves the injection of gas-filled microbubbles that do not extravasate. This...

Feb 20, 2018

Nanoparticle-uptake behavior in two standard cell lines, NIH/3T3 and A549

Etaluma

Feb 7, 2018

The uptake of nanomaterials into different cell types is a key issue for the determination of nanotoxicity as well as...

Mapping and phasing of structural variation in patient genomes using nanopore sequencing

Sage Science

Feb 6, 2018

Scientists used the Oxford MinION nanopore sequencer with a novel pipeline, NanoSV, to detect structural variants from...

De novo design of a biologically active amyloid

Mauna Kea Technologies

Feb 5, 2018

Short amyloidogenic protein fragment can induce the aggregation of a protein normally not associated with amyloidosis...

Feb 20, 2018

Exploration of melanoma metastases in mice brains using endogenous contrast photoacoustic imaging

VisualSonics FujiFilm

Feb 2, 2018

This recent article by Lavaud et al., uses high-frequency ultrasound and spectroscopic photoacoustic imaging as a...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Feb 12, 2018

Current methods for assessing the growth and shrinkage  of  tumor  spheroids  are  limited  by  one  or  more  of  the  following:  (1)  assay  workflows  that  are  time-consuming,  expensive  or  laborious,  (2)  a  requirement  to  label  the  cells  (e.g.  a  fluorescent  probe)  which  may  perturb  the  biology  and  may  not  be  amenable  to  primary  tissue,  (3)  single  time-point  readouts  that  do  not  report  the  full  timecourse,  (4)  indirect  readouts (e.g. ATP) that may overlook  valuable morphological insight and/or mis-report cell growth.In  this  application  note  we  describe  methods  and  validation  data  for  miniaturized  (96/384-well)  live-cell  tumor  spheroid  assays   that   are   based   on   non-invasive   bright-field   image   analysis  performed  with  the  IncuCyte  S3  Spheroid  software  module.  Tumor  spheroids  are  formed  in  ultra-low  attachment  (ULA) plates and monitored for size and morphology for up to 2  weeks.  These  assays  are  flexible,  simple  to  run  and  provide  automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Application
Product news

Get more info

Riccardo Pasculli

Field Application Specialist

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

Real-time automated measurements of cell health, movement and function, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey