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ACEA webinar series: Using xCELLigence RTCA to study biofilms formation

Acea Biosciences

Feb 14, 2018

xCELLigence instruments are revolutionizing pure and applied biofilm research. Beyond gains in efficiency, the...

NovoCyte live technical webinar: a flow cytometry method for detecting bacteria in water

Acea Biosciences

Feb 9, 2018

Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible...

Studying bacterial biofilm formation and stress responses using the BioFlux microfluidic platform

Fluxion Biosciences

Dec 14, 2017

Dr. Webber is a group leader at the Quadrum Institute and a leading authority on antimicrobial resistance. Recently,...

Imaging of infection and tumors using labelled siderophores

Bruker Biospin

Nov 15, 2017

In this webinar Zbynek Novy and Milos Petrik from the Institute of Molecular and Translational Medicine at Palacky...

Mar 19, 2018

Live technical webinar: measuring Calcium Flux by flow cytometry

Acea Biosciences

Sep 14, 2017

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+...

Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity


Aug 21, 2017

The success of immune checkpoint blockade adds a new therapeutic category to the cancer therapy repertoire. Despite...

Deep inside biological mechanisms: Ultra-high force resolution and correlated fluorescence


Jul 19, 2017

The C-Trap is the world’s first instrument that combines optical tweezers, confocal microscopy or STED nanoscopy and an...

Mar 19, 2018

Real-time analysis of cellular phagocytosis - Tuesday, June 20, 2017, 5 pm CEST


Jun 1, 2017

To identify new CD47 and phagocyte modulators, novel and validated assays with throughput commensurate to drug...

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Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing

Jul 10, 2017

Wednesday, July 12, 2017, 9 pm CEST.

Dan MacLeod Ph.D., Precision BioSciences, Inc.  

At Precision BioSciences, we have developed an allogeneic method using our proprietary ARCUS gene-editing technology to generate “universal” CAR T cells from healthy donors. These cells are engineered to eliminate expression of the endogenous T cell receptor making them safe for transfer to unrelated patients. By integrating the CAR transgene at a single genomic site, we can tightly control the level of CAR expression on the cell surface. We further optimize the cells by designing new CAR T constructs that include modifications to the extracellular binding domains as well as the intracellular signaling domains. Evaluating the impact of these new modifications requires sensitive methods to assay CAR T cell function. Traditional methods for evaluating cytotoxicity cannot effectively predict in vivo activity of a CAR T product; this is because they assess short-term killing potential rather than the ability to expand, persist, and exhibit serial killing. We have used the ACEA xCELLigence instrument for both B Cell Killing Assays and Leukemic Cell Killing Assays to test multiple CAR constructs with modified ectodomains, endodomains, and other modifications, and we have found this platform sensitive enough to detect subtle yet significant differences in performance not observed in traditional killing assays. Furthermore, the sensitivity of the instrument has enabled us to evaluate CAR constructs using cell numbers that are orders of magnitude lower than other assays, greatly enhancing our throughput and capabilities.

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Related technologies: Real-time, Label Free Cell Analysis

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Riccardo Pasculli

Field Application Specialist



+420 731 127 717

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Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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A unique, portable, impedance-based system for cell-based assays, such as cell growth, proliferation, cytotoxicity, adhesion and morphological dynamics

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

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We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic