Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Automated microscopy and Spatial Proteomics
Real-time, label free cell analysis
Nano and micro particle analysis
Accelerate to discover
Related topics
Discover the Celloger live-cell analyzers : miniaturized and powerful tools for every Biologist
Apr 5, 2024
Celloger live-cell analyzers, image and analyze your cells in real-time, inside your incubator. With its exceptional...
The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging
Apr 4, 2024
Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging...
A microthrombus-driven fixed-point cleaved nanosystem for preventing post-thrombolysis recurrence
Apr 3, 2024
A thrombin-responsive and fixed-point cleaved Fu@pep-CLipo was developed for highly efficient and precise thrombolysis...
Webinar: Multimodal Assessment of Hypoxia in Tumors: From the Lab to the Clinic
Apr 2, 2024
25 April 2024, 4PM CET
This webinar will be of interest to multiple profiles in the community of biomedical...
Multiplexed tissue imaging using the Orion platform to reveal the Spatial Biology of Cancer
Mar 27, 2024
In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable...
Cytek SpectroFlo Software Version 3.3 has been released!
Mar 25, 2024
SpectroFlo Software Version 3.3 has been released, enabling new assays with SpectroFlo Software version 3.3, including...
A 19-color single-tube Full Spectrum Flow Cytometry for the detection of Acute Myeloid Leukemia
Mar 13, 2024
This recent publication in Cytometry Part A describes the development and comprehensive workflow of a single-tube,...
18F-labeled somatostatin analogs for somatostatin receptors (SSTRs) targeted PET imaging of NETs
Mar 11, 2024
A novel 18F-radiolabeled somatostatin analogue, [Al18F]NODA-MPAA-HTA, was synthesized and evaluated for positron...
Jun 9, 2017
The ability to quantitatively correlate T cell biomarker expression/secretion with target cell killing is critical in both basic and applied studies of tumor immunology. Coupling flow cytometry with multiplex bead-based immunoassays enables quantitative measurement of multiple secreted targets in a single sample simultaneously. In this Application Note, we demonstrate a workflow involving the use of an xCELLigence Real-Time Cell Analysis (RTCA) instrument in conjunction with a NovoCyte flow cytometer to study both CTL-mediated destruction of tumor cells and the corresponding secretion of cytokines and cytolytic proteins by CTLs. Representing a promising new class of therapeutics, bispecific T-cell engagers (BiTEs) harness the power of the adaptive immune response by enhancing the ability of CTLs to specifically recognize and eliminate tumors. For example, CD19-BiTEs are designed to simultaneously bind CD3 on CTLs and CD19 on cells of B cell lineage, thereby enhancing the efficiency of CTL effector functions against various B cell derived tumors. Herein, the enhancement effects of CD19-BiTEs have been evaluated using three distinct assays. While target cell death was monitored using an RTCA impedance assay and a flow cytometry-based cytolysis assay, secretion of cytokines and cytolytic proteins was quantified in a bead-based multiplex flow cytometry assay.
Related technologies: Conventional flow cytometry
Get more info
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com