Cellular analysis

3D cell culture rotary system
Miroslav Hutňan

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Miroslav

Hutňan

+420 739 394 360

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3D cell culture rotary system

Rotary cell culture system technology brings cultured cells closer to native tissue

In the late 1980's, scientists and engineers at NASA’s Johnson Space Center developed a new type of cell culture system. The system was based on the principle of clinorotation, defined as the nullification of the force of gravity by slow rotation about one or two axes. The clinostat developed at NASA was a single axis device known as the Rotating Wall Vessel (RWV).

The original purpose of the RWV was to simulate the effects of microgravity in order to study the effects of weightlessness at a cellular and molecular level in a ground-based system. It was quickly noted that when anchorage-dependent cells were cultured in the RWV, they formed three-dimensional aggregates that resembled in vivo tissue. In subsequent years many investigators also demonstrated that these 3D aggregates were functionally more similar to native tissues than cells cultured in conventional 2D cell culture technology. The basic elements that govern the cell culture environment in the RWV conducive to 3D cell culture are the rotational speed of the vessel and the mass transfer of nutrients in the media.

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3D cell culture rotary system products

The RCCS-SC systems are a unique 3D cell culture technology with scaled down size for economic and more efficient stem cells growth.

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RCCMAX allows undisturbed 3D cultivation of cells in vitro condition with continuous supplementation of fresh media and oxygen.

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The autoclavable RCCS use culture vessels designed for repeated autoclaving, and ideal for long-term use. Suitable for any of the Synthecon culture vessels supplied with variable speed motor drives equipped with tachometer.

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The disposable (single use) vessel culture systems are low cost, easy to use, rotary bioreactor systems that utilize disposable culture vessels to enable 3D cell culture.

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