Molecular technologies

Automated Single-cell Genomics
Regina Fillerová

Application Specialist

Regina

Fillerová

+420 731 127 718

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Automated single-cell genomics

The Revolution at Hand

 

Individual cells can differ by size, protein levels, and expressed mRNA transcripts, even within nominally homogeneous cell populations. Therefore, the tacit assumption that every cell in your sample behaves exactly the same way is a dangerous gamble; taking averages of pooled cells can mask the dramatic variations in gene expression among cells. Recognizing cellular variations in what appear to be homogenous populations has become crucial to advancing stem cell research, understanding cancer cells, identifying immune responses, studying the effectiveness of biological therapies, and discovering the mechanisms of neurodegenerative diseases.

Geneticists and clinical researchers have been looking for a complete workflow that can examine and differentiate individual cells and group them according to unique genomes and transcriptomes while minimizing technical noise.

The innovative C1 system carefully isolates single cells into individual reaction chambers in exclusive integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for qPCR or sequencing analysis—all in as little as four hours.  

The technology allows achieving highly reliable and reproducible data from individual cells with microfluidic IFCs, designed to capture cells from 5 to 25 µm. In combination with dedicated ready-to-use reagent solutions for DNA sequencing, mRNA sequencing and targeted gene expression, it fully automates the single-cell research.

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Automated Single-cell Genomics products

The first automated solution for single-cell genomics, now capable of even more

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The system for precise manipulation of biological pathways for in vitro cell culture.

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Polaris actively selects single cells from heterogeneous cell samples and holds them in individual, environmentally controlled reaction chambers.

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