Molecular technologies

Single-molecule Interactions
Miroslav Hutňan

Sales Manager

Miroslav

Hutňan

+420 739 394 360

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Single-molecule Interactions

Live observation and measurement at the molecular level

Real-time evaluation of genetic and proteomic processes at the molecular level is key for understanding the events leading to cancer and other genetic process related diseases. Current state-of-the-art technologies life science research are able to determine the structure of proteins with near-atomic resolution, based on which models are derived for their function and dynamic behaviour. Making live interaction of proteins on the DNA trackable in real time, with single molecule resolution and under natural conditions presents a great step ahead.

Optical tweezers are scientific instruments using highly focused laser beams to provide an attractive or repulsive force, depending on the refractive index difference between the target and its surroundings.  The force is usually on the order of piconewtons, used to physically hold and move microscopic dielectric objects. The beam is typically focused by sending it through a microscope objective. The narrowest point of the focused beam contains a very strong electric field gradient and the dielectric particles are attracted along the gradient to the region of strongest electric field, i.e, the center of the beam.

Acoustic Force Spectroscopy is a single-molecule, lab-on-a-chip technology in which acoustic waves (ultrasound) are employed to exert forces from sub-pN to hundreds of pNs on thousands of biomolecules (such as DNA, RNA or proteins) in parallel, with sub-millisecond response time and inherent stability.  AFS technology consists of a glass micro-fabricated microfluidic chip and includes transparent piezoelectric transducers that generate acoustic waves. AFS enables scientist to investigate structure-function of proteins, new biological mechanisms, compound screening and cell mechanics. In addition, free energy diagrams, kinetic rates and intermediate states in reaction pathways can also be studied and identified.

Quartz Crystal Microbalance (QCM) technology offers an innovative platform to study molecular interactions thanks to its independence of proximity, size and optical parameters, low non-specific binding surfaces and optimized fluidics. An applied AC-potential causes a quartz crystal to vibrate at its resonance frequency. As molecules flown over the crystal bind to the surface, the vibration frequency changes. The difference in frequency is direct proportional to the change in mass and is used to characterize label-free molecular interaction in real time. The QCM technology can be used to measure label-free biomolecules and macrostructures such as antibodies, proteins, carbohydrates, cells, DNA, bacteria, viruses and more.

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Single-molecule Interactions products

Live, simultaneous and correlative visualization and manipulation of molecular interactions with sub-picoNewton force resolution and sub-nm position resolution.

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Acoustic Force Spectroscopy is a single-molecule, lab-on-a-chip technology in which acoustic waves are used to exert forces on thousands of biomolecules.

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The u-Flux microfluidic system is an integrated solution to reliably perform single-molecule experiments in a laminar-flow environment.

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Dual channel label-free biosensor capable of handling crude assays and sera.

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The label-free biosensor that combines biochemical and cell-based assays.

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