Accelerate to discover

Back to filter

Related topics

Cytek webinar : Evaluating Spectral Cytometry for Immune Profiling in Viral Disease

Cytek Biosciences

Apr 19, 2021

In conventional fluorescence cytometry, each fluorophore in a panel is measured in a target detector, through the use...

Rethinking 3D cell culture with IncuCyte: advanced cell cultures from iPSCs and organoids

Sartorius

Apr 14, 2021

The world is neither flat nor two-dimensional, so why should cell culture be? Traditional cell culture systems grow...

The impact of environmental Oxygen content on Natural Killer Cell Immunotherapy

Xcell Biosciences

Apr 13, 2021

Natural Killer (NK) cells are being studied in the clinic in a number of settings, including solid tumor...

Observe the movement of live cells that make up living tissue in vivo

IVIM Technology

Apr 8, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of live cells that make up...

Apr 20, 2021

Cytek webinar recording : Improved flow cytometry assay resolution with autofluorescence extraction

Cytek Biosciences

Mar 30, 2021

The power of autofluorescence extraction in the detection of dim fluorescence on highly autofluorescent cells, and the...

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Apr 20, 2021

Bruker webinar : High-resolution exploration of ESM-based vascularized tissue-flaps

Bruker Biospin

Mar 22, 2021

By employing a tissue engineering approach based on biological tissue constructs, Dr. Redenski has fabricated composite...

Show all topics (10)

3D time lapse imaging and quantitative analysis of multilayered Endothelial cells migration

Oct 22, 2020

Yokogawa CSU (confocal scanner unit) is a confocal unit using a microlens-enhanced dual Nipkow disk confocal optical system, which has been shown to be less harmful to living cells compared to conventional single beam scanning devices. The CQ1 is an all-in-one confocal quantitative imaging cytometer based on the CSU. Here we report the 3D time lapse live cell imaging in a multilayered cell sheet using CQ1.

Methods:

  • Five-layered myoblast cell sheets were constructed from human skeletal muscle myoblasts (HSMM) and human skeletal muscle fibroblasts (HSMF)
  • HSMMs and HSMFs were labeled with CellTrackerTM Orange
  • Human umbilical vein endothelial cells (HUVEC) expressing GFP (GFP-HUVEC) were overlaid by the cell sheet and co-cultured.
  • Time lapse imaging (67 hours, 30 min interval, 40x objective lens , 49 fields) was performed by CQ1 equipped with an internal incubation chamber to regulate culture environment.

Read more!

Technical breakthrough
Application

Related technologies: Live cell imaging

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Yokogawa

Yokogawa is a leading provider of confocal quantitative live cell imaging platforms. Combining superior technology with engineering services, project management, and maintenance, Yokogawa delivers field proven operational efficiency, safety, quality, and reliability. 

Related products

Confocal quantitative image cytometer CQ1 offers a new approach to image quantification

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey