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A novel high throughput assay for diagnosis by real-time monitoring of parasite motility

Jun 7, 2017

Here we describe a novel application for a real-time cell monitoring device (xCELLigence) that can simply and objectively assess anthelmintic effects by measuring parasite motility in real time in a fully automated high-throughput fashion. We quantitatively assessed motility and determined real time IC50 values of different anthelmintic drugs against several developmental stages of major helminth pathogens of humans and livestock, including larval Haemonchus contortus and Strongyloides ratti, and adult hookworms and blood flukes. The assay enabled quantification of the onset of egg hatching in real time, and the impact of drugs on hatch rate, as well as discriminating between the effects of drugs on motility of drug-susceptible and –resistant isolates of H. contortus.

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Scientific paper

Related technologies: Real-time, label free cell analysis

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Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

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