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Homepage>Discover>A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

May 9, 2019

The Bruker PET/CT Si78 now pairs total-body PET and SkyScan microCT technology. This combination offers the advanced...

Cell by cell lung cancer marker profiling using high-parameter spectral flow cytometry

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In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model

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As a trace element nutrient, cobalt is critical for both prokaryotes and eukaryotes. In the current study, a turn-on...

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Layer-by-layer assembly techniques have been extensively studied in cell biology because of their simplicity of...

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Modified Beta-carotene-bound albumin nanoparticles for breast tumor ablation

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The albumin nanoparticles were fabricated using a beta-carotene cross-linker via the Nab technique. These new albumin...

Spectral flow cytometric analysis of loss-of-function mutations in granular cell tumors

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Cell plasticity associated to circulating tumor cells from prostate cancer patients

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Treatment resistance is associated with tumor cells molecular plasticity. In metastatic castration resistant prostate...

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A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Feb 19, 2018

Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.

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Scientific paper

Related technologies: Conventional flow cytometry

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