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Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

The Role of Micro-CT in Imaging Breast Cancer Specimens

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

Sep 19, 2020

Immunophenotyping Extracellular Vesicles using Amnis CellStream

May 29, 2020

Attempts to analyze EVs using traditional PMT- based flow cytometers has been hampered by the limit of detection of...

Four-week individual caging of male ICR mice alters body composition without change in body mass

May 25, 2020

Could individual caging affect the physical composition of outbred mice? To investigate this, dual X-ray absorptiometry...

Detect structural variants at 1% allele fraction: Dive deeper into heterogeneous cancer samples

May 22, 2020

To get a complete picture of highly rearranged cancer genomes in heterogeneous samples, use Bionano genome imaging...

Sep 19, 2020

Insulin receptor substrate 1 is a substrate of the Pim protein kinases

May 18, 2020

The goal of these studies was to identify Pim substrate(s) that could help define the pathway regulated by these...

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A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Feb 19, 2018

Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.

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Scientific paper

Related technologies: Conventional flow cytometry

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