Accelerate to discover

Homepage>Discover>A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Back to filter

Related topics

Small animal 13C-hyperpolarized MRI

Feb 8, 2019

13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

Jan 30, 2019

Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Jan 17, 2019

In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. The...

Improved differentiation of Retinal Organoids stem cells derived using rotating-wall vessels

Jan 15, 2019

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a...

Feb 20, 2019

CT‐analyzer plug‐ins: ROI shrink‐wrap and primitive ROI

Jan 7, 2019

The ROI shrink‐wrap gives the possibility to automatically generate a region‐of‐interest by adapting to the shape of...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Dec 17, 2018

MRI is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

Feb 20, 2019

Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies

Dec 6, 2018

Accurate labeling of endogenous proteins for advanced light microscopy in living cells remains challenging. Nanobodies...

Show all topics (10)

A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Feb 19, 2018

Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.

Read more

Scientific paper

Related technologies: Conventional flow cytometry

Get more info