Accelerate to discover

Back to filter

Related topics

Saphyr identifies all variants detected by the standard of care in cytogenetics

BioNano Genomics

Jun 1, 2020

Cancer genomics consortium members demonstrate 100% concordance between Bionano Genomics optical mapping technology and...

Immunophenotyping Extracellular Vesicles using Amnis CellStream

Cytek Biosciences

May 29, 2020

Attempts to analyze EVs using traditional PMT- based flow cytometers has been hampered by the limit of detection of...

Four-week individual caging of male ICR mice alters body composition without change in body mass

MEDIKORS

May 25, 2020

Could individual caging affect the physical composition of outbred mice? To investigate this, dual X-ray absorptiometry...

Detect structural variants at 1% allele fraction: Dive deeper into heterogeneous cancer samples

BioNano Genomics

May 22, 2020

To get a complete picture of highly rearranged cancer genomes in heterogeneous samples, use Bionano genome imaging...

Jun 3, 2020

The qNano Gold: Obtain the most detailed Information for each individual nanoparticle

IZON

May 20, 2020

The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful...

Insulin receptor substrate 1 is a substrate of the Pim protein kinases

Spectral Instruments Imaging

May 18, 2020

The goal of these studies was to identify Pim substrate(s) that could help define the pathway regulated by these...

Webinar recording: Isolating ultra-high molecular weight (UHMW) DNA for genome analysis

BioNano Genomics

May 12, 2020

Watch the webinar from Bionano Genomics and learn the tips and tricks for isolating high quality UHMW DNA - right from...

Jun 3, 2020

Implementation of Micro CT in CAD/CAM dentistry for image processing and soft computing

Bruker Biospin

May 4, 2020

Present radiology devices are playing an important role in clinical dentistry but their area of application islimited...

Show all topics (10)

A novel real time imaging platform to quantify Macrophage Phagocytosis

Feb 26, 2018

 Quantification of macrophage phagocytosis in vitro has traditionally been technically challenging. Here we report the optimization and validation of the IncuCyte S3 real time imaging platform for macrophage phagocytosis based on pHrodo pathogen bioparticles, which only fluoresce when localized in the acidic environment of the phagolysosome. Image analysis and fluorescence quantification were performed with the automated IncuCyte Basic Software. Titration of the bioparticle number showed that the system is more sensitive than a spectrofluorometer, as it can detect phagocytosis when using 20× less E. coli bioparticles. We exemplified the power of this real time imaging platform by studying phagocytosis of murine alveolar, bone marrow and peritoneal macrophages. We further demonstrate the ability of this platform to study modulation of the phagocytic process, as pharmacological inhibitors of phagocytosis suppressed bioparticle uptake in a concentration-dependent manner, whereas opsonins augmented phagocytosis. We also investigated the effects of macrophage polarization on E. coli phagocytosis. Bone marrow-derived macrophage (BMDM) priming with M2 stimuli, such as IL-4 and IL-10 resulted in higher engulfment of bioparticles in comparison with M1 polarization. Moreover, we demonstrated that tolerization of BMDMs with lipopolysaccharide (LPS) results in impaired E. coli bioparticle phagocytosis. This novel real time assay will enable researchers to quantify macrophage phagocytosis with a higher degree of accuracy and sensitivity and will allow investigation of limited populations of primary phagocytes in vitro.

Read more

 

 

Scientific paper
Application
Video

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey