Accelerate to discover

Back to filter

Related topics

Webinar: Join us for an In-Depth Look at the Revolutionary Small Animal Radiotherapy System at STTARR

Precision X-Ray

Aug 17, 2023

Register for an engaging webinar led by Naz Chaudary, Ph.D., and Research Technician, Alex Wang from the...

Optimizing Gene Expression with Bioluminescence & the piggyBac System

Spectral Instruments Imaging

Aug 15, 2023

Discover how bioluminescence imaging & the piggyBac gene editing system optimize & track gene expression in mouse...

High-plex immunofluorescence imaging and traditional histology of the same tissue section

RareCyte

Aug 7, 2023

RareCyte Orion’ platform has been used for collecting H&E and high-plex immunofluorescence images from the same cells...

Drive key insights and discoveries with NEW Live Cell Analysis applications for eSight system

Agilent technologies

Aug 2, 2023

eSight is now powerful like never before. Learn more about new dedicated application modules for live-cell analysis: 3D...

Sep 30, 2023

Single cell-resolution in situ sequencing elucidates spatial dynamics of multiple sclerosis

Vizgen

Jul 14, 2023

MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, fluidics, image processing,...

MARS - High Efficiency Separation of CD34+ HSC from Mobilized Blood

Applied Cells

Jul 12, 2023

MARS platform provides an easy and cost-effective protocol for CD34+ cell isolation. Single pass CD34+ HSC enrichment...

43 markers, ONE tube : Impress yourself with Cytek Aurora Spectral Cytometer

Cytek Biosciences

Jul 11, 2023

Using Cytek full spectrum flow cytometry, scientists at Hamad Medical Corporation, developed a 43 color panel to...

Sep 30, 2023

InAlyzer - The technology chosen by NASA

MEDIKORS

Jul 10, 2023

MEDIKORS's InAlyzer was finally selected as the equipment to be used for NASA's space environment biological research

Show all topics (10)

Adhesion-based simple capture and recovery of circulating tumor cells using CQ1 imaging cytometer

Jan 24, 2018

Here, we explore the possibility of collecting CTCs using blood-compatible and thermo-responsive poly(2-(2-ethoxyethoxy) ethyl acrylate-co-2-(2-methoxyethoxy) ethyl methacrylate) (P(Et2A-Me2MA)) through adhesion and detachment by incubation under a lower critical solution temperature of P(Et2A-Me2MA). A P(Et2A-Me2MA)-coated substrate is dissolved by incubation under 15 °C. A P(Et2A-Me2MA)-coated substrate can suppress platelet adhesion whereas it allows the cancer cells to adhere by an epithelial cell adhesion molecule (EpCAM) expression-independent mechanism. These results suggest that cancer cells can specifically adhere to a P(Et2A-Me2MA)-coated substrate, which can be used to isolate CTCs from peripheral blood. Moreover, approximately 90% of the adherent cells can be detached by incubation at 10 and 15 °C for 30 and 90 min, respectively. The collected cells can be cultured healthily in the presence of dissolved P(Et2A-Me2MA), suggesting that the cytotoxicity of P(Et2A-Me2MA) is low. In conclusion, P(Et2A-Me2MA) is suitable for the development of devices that collect intact CTCs via an adhesion-based method.

Read more

 

Scientific paper
Application

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Yokogawa

Yokogawa is a leading provider of confocal quantitative live cell imaging platforms. Combining superior technology with engineering services, project management, and maintenance, Yokogawa delivers field proven operational efficiency, safety, quality, and reliability. 

Related products

Confocal quantitative image cytometer CQ1 offers a new approach to image quantification

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey