Accelerate to Discover

Back to filter

Related topics

Early fetal sex determination using micro-volume of maternal plasma

BJS Biotechnologies

Jun 16, 2018

 A qPCR-based assay, SneakPeek Early Gender Test (Gateway Genomics) has been developed to determine fetal sex as early...

3R Compliant drug development studies

Bruker Biospin

Jun 8, 2018

In preclinical imaging, magnetic resonance imaging (MRI) can be an attractive alternative to researchers who have...

Kinase study of cytotoxicity voted paper of the year.

Acea Biosciences

Jun 6, 2018

Society of Toxicology voted a xCELLigence based paper the Paper of the Year. The study of cytotoxicity was done using...

Heterodimeric compounds with dual-targeting active against both HIV and tuberculosis.

Acea Biosciences

Jun 4, 2018

Search for dual action drugs against both HIV and Tuberculosis has lead to the creation of heterodimeric compounds with...

Jun 18, 2018

Mutual destruction of deep Lung tumor tissues by nanodrug‐conjugated and CQ1 imaging cytometer

Yokogawa

Jun 1, 2018

Lung cancer is a highly malignant tumor, and targeted delivery of anti‐cancer drugs to deep lung tumor tissue remains a...

Method to quantify local force distribution within biomolecular systems

LUMICKS

May 24, 2018

The journal Nano Letters has recently published the article “Quantifying Local Molecular Tension Using Intercalated DNA...

Bionano fall user workshop

BioNano Genomics

May 23, 2018

We are happy to announce the Bionano Genomics’ 2018 user workshop taking place in San Diego on October 15-16,...

Jun 18, 2018

Registering FDG/PET mouse and AMYViD/PET-CT rat studies to brain VOI atlases

Bruker Biospin

May 22, 2018

Preclinical NeuroPET is employed widely in studies of stroke, ischemia, addiction and eurodegenerative diseases. In...

Show all topics (10)

AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

Read more!

Application
Product news

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Field Application Specialist

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey