Accelerate to discover

Back to filter

Related topics

IncuCyte webinar: Advances in 3D cell models for cancer biology

Sartorius

Jul 1, 2020

Get answers to your questions on 3D spheroid-based drug discovery, organoid research and working with advanced cell...

Bruker MicroCT systems supporting suality control for refurbished FFP masks

Bruker Biospin

Jun 29, 2020

In the effort to tackle the coronavirus pandemic, Bruker is proud that its instruments are being used to ensure quality...

Advance your Neuro-Oncology applications with IncuCyte

Sartorius

Jun 25, 2020

Neuro-oncology research can benefit from robust translational in vitro models to gain greater understanding of brain...

MicroCT Applications in Lung research and Immunology

Bruker Biospin

Jun 22, 2020

The ability to monitore and quantitate lung disease in real time is an advantage to using small animal microCT. Also,...

Jul 3, 2020

Imaging Extracellular Vescicles with super-resolution microscopy

ONI

Jun 18, 2020

Extracellular vesicles (EVs) play key roles in cell-to-cell communication. EVs can cross biological barriers (such as...

The Role of Micro-CT in Imaging Breast Cancer Specimens

Bruker Biospin

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Cytek Northern Lights: 41 parameters with 3 lasers only

Cytek Biosciences

Jun 12, 2020

Cytek Biosciences Northern Lights incorporates the same innovations as the Aurora, but at a lower price point. It...

Jul 3, 2020

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Bruker Biospin

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

Show all topics (10)

AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

Read more!

Application
Product news

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey