Accelerate to discover

Back to filter

Related topics

Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

Sage Science

Aug 16, 2019

The BluePippin was used for High Pass DNA size selection prior to library construction. ONT Promethion was used for...

Fluxion Biosciences launches custom liquid biopsy NGS panel development

Fluxion Biosciences

Aug 14, 2019

Fluxion a leading developer of precision solutions for life science research and diagnostics, announced that can...

Superior flow cytometry data with NEW Curiox Laminar Wash systems - centrifuge - less washing

Curiox

Aug 12, 2019

The Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry....

Electroporation using Lumascope: A sustainable method for Adipogenous Mesenchymal Stem Cells

Etaluma

Aug 7, 2019

Human mesenchymal stem cells derived from adipose tissue (AD-hMSCs) represent a promising source for tissue engineering...

Aug 19, 2019

The best way to fragment DNA for genomic and paired end library construction

Digilab

Aug 5, 2019

Digilab NextGen Shear DNA Shearing offers the simplest, most reproducible, and most controllable method available for...

New Cytek Full Spectrum Viewer tool, a powerful guide for your fluorochrome choices

Cytek Biosciences

Aug 2, 2019

Want to see which fluorochromes have unique signatures and which are identical? Then the Cytek Full Spectrum Viewer...

Software Update: Bionano releases a new pipeline for low Aalelic fraction SVs

BioNano Genomics

Jul 31, 2019

Bionano Solve v3.4 is a major update that introduces an entirely new pipeline to detect SVs at low allelic fraction and...

Aug 19, 2019

On-cartridge preparation and evaluation of 68Ga-, 89Zr- and 64Cu-precursors for cell radiolabelling

Bruker Biospin

Jul 29, 2019

Method of on-cartridge formation of gallium-68, zirconium-89 and copper-64 complexes in small volumes suitable for cell...

Show all topics (10)

AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

Read more!

Application
Product news

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey