Accelerate to discover

Back to filter

Related topics

Zebrafish heart imaging

Bruker Biospin

Nov 14, 2019

Today watch this interesting video interview with Anabela Bensimon-Brito, Department of Developmental Genetics, Max...

Webinar recording: Analysis of extracellular vesicles by AMNIS imaging flow cytometer

Luminex

Nov 4, 2019

Although conventional flow cytometry is limited to measure small particles down to approximately 300 nm – 500 nm, an...

Targeted mitochondrial DNA extraction and enrichment using the SageHLS

Sage Science

Oct 31, 2019

Sage Science introduced a novel approach for enriching mitochondrial DNA for sequence analysis without PCR or gradient...

Join the webcast: Addressing cancer gene therapy efficacy with IncuCyte Live–Cell Imaging

Sartorius

Oct 29, 2019

During this webinar, Dr Pierre Cordelier will discuss the use of IncuCyte Live-Cell Imaging and Analysis, to track gene...

Nov 14, 2019

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer at a single low dose

Spectral Instruments Imaging

Oct 24, 2019

Pancreatic ductal adenocarcinoma (PDAC) has been increasing by 0.5% per year in the United States. This study describes...

Bionano ICS software upgrade: 2600 Gbp with new instrument control software for Bionano Saphyr

BioNano Genomics

Oct 24, 2019

Upgrade to Instrument Control Software v4.9 for higher throughput. Saphyr Instrument Control Software (ICS) v4.9 is the...

New code dedicated to esophageal procedures with Confocal Laser Endomicroscopy

Mauna Kea Technologies

Oct 21, 2019

Announcement of the creation of a new specific procedural code to be added to the Common Classification of Medical...

Nov 14, 2019

Flow cytometry technology in an affordable instrument - Discover Guava Muse

Luminex

Oct 21, 2019

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava Muse cell analyzer, you can now...

Show all topics (10)

AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

Read more!

Application
Product news

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey