Accelerate to discover

Back to filter

Related topics

Quantitative characterization of metaphase cellular events using 3D imaging cellular events 

Yokogawa

Sep 20, 2018

In conventional monolayer cell culture systems, cell nuclei show dynamic and cyclic changes in theirshape and height....

A carboxymethyl dextran-based polymeric conjugate as the antigen carrier for cancer immunotherapy

Spectral Instruments Imaging

Sep 18, 2018

A polymeric conjugate, composed of a carboxymethyl dextran (CMD) as the backbone and ovalbumin (OVA) as a model foreign...

Adenovirus type B detection using the xxpress qPCR thermal cycler

BJS Biotechnologies

Sep 17, 2018

The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...

Contrast-enhanced ultrasound imaging

VisualSonics FujiFilm

Sep 5, 2018

Are you interested in photoacoustic imaging and would like to enhance your contrast imaging acquisitions? Just use...

Sep 21, 2018

BluePippin-DNA size selection for NGS with pulsed-field (100bp-50kb)

Sage Science

Sep 3, 2018

Highly sensitive detection of mutations in CHO cell recombinant DNA uses multi‐parallel single molecule real‐time DNA...

Diagnosis of Acute Cellular Rejection Using Probe-Based Confocal Laser Endomicroscopy in Lung

Mauna Kea Technologies

Sep 3, 2018

Mauna Kea Technologies announced the publication of a prospective multicenter study that demonstrates the potential of...

Low shear micro-gravity, a better way to do 3D cell culture

Synthecon

Aug 20, 2018

A variety of technologies for cell and tissue culture have been developed since the first use of glass or plastic...

Sep 21, 2018

New technologies to uncover the molecular basis of disorders of sex development

BioNano Genomics

Aug 16, 2018

Newer approaches, such as whole-genome sequencing and whole-genome mapping have the potential to identify novel...

Show all topics (10)

Bronchoscopic confocal fluorescence microscopy for in vivo assessment of free-breathing mice

May 3, 2018

Respiratory diseases, such as pulmonary infections, are an important cause of morbidity and mortality worldwide. Preclinical studies often require invasive techniques to evaluate the extent of infection. Fibered confocal fluorescence microscopy (FCFM) is an emerging optical imaging technique that allows for real-time detection of fluorescently labeled cells within live animals, thereby bridging the gap between in vivo whole-body imaging methods and traditional histological examinations. Previously, the use of FCFM in preclinical lung research was limited to endpoint observations due to the invasive procedures required to access lungs. Here, we introduce a bronchoscopic FCFM approach that enabled in vivo visualization and morphological characterisation of fungal cells within lungs of mice suffering from pulmonary Aspergillus or Cryptococcus infections. The minimally invasive character of this approach allowed longitudinal monitoring of infection in free-breathing animals, thereby providing both visual and quantitative information on infection progression. Both the sensitivity and specificity of this technique were high during advanced stages of infection, allowing clear distinction between infected and non-infected animals. In conclusion, our study demonstrates the potential of this novel bronchoscopic FCFM approach to study pulmonary diseases, which can lead to novel insights in disease pathogenesis by allowing longitudinal in vivo microscopic examinations of the lungs.

Do you want to know more? Read this interesting article!

Scientific paper
Application

Related technologies: Probe based confocal microscopy

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 737 202 127

Send Message

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio Lab is a single band fiber based microscope for observing in real time at cellular scale

show detail

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey