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Webinar: Join us for an In-Depth Look at the Revolutionary Small Animal Radiotherapy System at STTARR

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Register for an engaging webinar led by Naz Chaudary, Ph.D., and Research Technician, Alex Wang from the...

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eSight is now powerful like never before. Learn more about new dedicated application modules for live-cell analysis: 3D...

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High speed, compact and fully automated microscope inside your incubator? Lumascope850

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The powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and...

MARS - High Efficiency Separation of CD34+ HSC from Mobilized Blood

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43 markers, ONE tube : Impress yourself with Cytek Aurora Spectral Cytometer

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Cancer immunotherapy: Macrophage-mediated phagocytosis

Oct 27, 2016

In a recent study the secreted glycoprotein thrombospondin 1 (TSP1) was shown to be a positive modulator of innate antitumor immunity by increasing M1 macrophage recruitment and stimulating reactive oxygen species (ROS)-mediated tumor cell killing. These conclusions were drawn, in part, by using xCELLigence RTCA impedance monitoring to evaluate the effect of TSP1 on macrophage/ monocyte activity when co-cultured with MDA-MB-231 breast adenocarcinoma target cells. The % cytolysis data clearly indicate that the tumoricidal activity of both differentiated U937 human monocytes and activated ANA-1 murine macrophages  are enhanced in the presence of TSP1.

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Related technologies: Real-time, label free cell analysis

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Riccardo Pasculli

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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Located inside a tissue culture incubator, the RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

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