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Putting cells in a centrifuge for multiple washes at 500 x g is not a natural state for the cells. Remember:
• You are changing mRNA expression and phenotypic expression
• Cells can die or be damaged during pelleting
• Cells can die or be damaged from shear forces when re-suspending the pellet
• Starting with a low cell number may result in a loosely formed pellet
Here we demonstrate an example of human PBMC nuclei washed with centrifuge or Laminar Wash technology. Centrifuged samples have inconsistent retention, with examples of large clumps. Nuclei are more distinct when washed on Laminar Wash systems. Retention numbers with Laminar Wash systems are higher and more consistent.
Related technologies: Automated sample preparation
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Curiox Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry, eliminating the usage of the centrifuge.
More info at:
https://www.curiox.com/
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