Accelerate to discover

Back to filter

Related topics

Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids

Cellenion

Jun 21, 2022

spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

Jun 14, 2022

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Incucyte AI module: Advanced Label-Free Classification Analysis

Sartorius & Essen BioScience

Jun 6, 2022

Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

Jun 3, 2022

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

Jul 5, 2022

An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing

Sage Science

Jun 1, 2022

Recent article from February 2022 shoes how size selection improved viral read mapping by over 90% for begomoviruses.

Intoducing cellenONE: The revolutionary Single Cell isolator

Cellenion

May 25, 2022

A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

May 24, 2022

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Jul 5, 2022

Extracellular Vesicle Purity Enhanced by Gen 2 qEV Columns With Customised Proprietary Resin

IZON

May 4, 2022

Izon Science has announced the launch of Gen 2, the enhanced range of qEV columns for size-exclusion...

Show all topics (10)

Electroporation using Lumascope: A sustainable method for Adipogenous Mesenchymal Stem Cells

Aug 7, 2019

Scientists, using Etaluma Lumascope evaluated transient transfection through electroporation for cell labeling and compared it with lentiviral transduction for AD-hMSCs. In addition, we tested whether nonsense DNA or a reporter gene such as enhanced green fluorescent protein (EGFP) is the more suitable label for AD-hMSCs. Using electroporation, the transfection efficiency reached a maximal level of 44.6 ± 1.1% EGFP-positive cells after selective and expansive cultivation of the mixed MSC population, and was 44.5 ± 1.4% after gene transfer with Cyanin3-marked nonsense-label DNA, which remained stable during 2 weeks of nonselective cultivation (37.2 ± 4.7% positive AD-hMSCs). Electroporation with both nonsense DNA and pEGFP-N1 led to a slight growth retardation of 45.2% and 59.1%, respectively. EGFP-transfected or transduced AD-hMSCs showed a limited adipogenic and osteogenic differentiation capacity, whereas it was almost unaffected in cells electroporated with the nonsense-label DNA.

Read more!

Scientific paper
Application

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

Related products

The true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent LumaScope

show detail

The original single color 488nm excitation fluorescence inverted microscopy

show detail

Lumascope 620 offers a larger sensor and 3-color fluorescence for the most powerful microscope offered anywhere at this price

show detail

Blue, green & red fluorescence and walk-away automation

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey