Accelerate to discover

Back to filter

Related topics

AMI HTX installation in CEMEX Iasi, Romania

Spectral Instruments Imaging

Sep 30, 2022

Presentation movie

Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging

RareCyte

Sep 13, 2022

Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...

Lumascope 720 for CENEMED Project in Romania

Etaluma

Aug 24, 2022

LS720 is automated microscope inside CO2 incubator, coming with powerful cell analysis software, able to perform...

Cytek Aurora at CIVIS Summer School

Cytek Biosciences

Aug 23, 2022

accela's workshop during CIVIS project for spectral flowcytometry, using Aurora from Cytek

Oct 1, 2022

xCELLigence eSight - Imaging & Impedance in real-time inside your incubator

Agilent technologies

Aug 22, 2022

The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell...

Standardize tumor sample preparation and reduce background with Curiox laminar wash system

Curiox

Aug 10, 2022

Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell...

Developing a 43 color panel on Cytek Aurora using Laminar Wash for sample prep

Curiox

Aug 2, 2022

Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are...

Oct 1, 2022

Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids

Cellenion

Jun 21, 2022

spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....

Show all topics (10)

Enhance fluorescent signals of PE-conjugates using NovoCyte flow cytometer with 561nm laser

Aug 3, 2016

The advantages of using 561nm laser configured NovoCyte flow cytometers include: enhancing fluorescent signals of PE-conjugated antibodies with 561nm excitation and increasing sensitivity on detecting dim signals; eliminating or minimizing FITC to PE spillovers by independent excitation with 488nm and 561nm lasers; favoring the detection of multiple fluorescent proteins such as GFP, DsRed and mCherry; fulfilling multicolor analysis of variety of applications.

PE series of fluorochromes (PE, PE-Texas Red, PE-CF594, PE-Cy5, PE-Cy5.5 and PE-Cy7) have a primary absorption peak of 565nm with secondary absorption peaks at 496 and 545nm that can be excited by either the 488nm or 561nm lasers. However, higher excitation efficiency of 561nm laser results in brighter fluorescence signals and detection sensitivity, manifested by improved stain index and separation of positive from negative populations.

Read more

 

Scientific paper
Application
Product news

Related technologies: Conventional flow cytometry

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey