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HLS-CATCH: Cas-9 assisted gene purification

May 24, 2019

How it works:
>Intact cells or nuclei are loaded into SageHLS cassettes.
>Electrophoretic extraction leaves chromosomal-length genomic DNA immobilized in the sample well wall.
>Custom Cas9 cleavases perform enzymatic DNA processing.
>Size selection electrophoresis moves Cas9-cleaved target regions into the gel channel, away from uncut untargeted genomic DNA in the sample well.
>Products are localized by qPCR after electroelution from the gel channel.

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Related technologies: Targeted DNA/protein size collection

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Regina Fillerová

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Regina

Fillerová

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Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

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Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

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Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

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Automated size selection for 24 samples at a time.

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