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Vizgen webinar: spatial relationships in Developmental Biology

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MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, In this webinar we will...

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Single Cell Deposition: the cornerstone of Flow Cytometry for cellular analysis and manipulation

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Emulate in vivo conditions – introduce shear flow to your experiments with BioFlux system

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Single Cell Deposition: the cornerstone of Flow Cytometry for cellular analysis and manipulation

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Validation of an orthotopic non-small cell lung cancer mouse model to use in radiotherapy studies

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Identification and measurement of bacterial size using the ImageStream

Apr 27, 2016

In this note, we describe two bacterial studies performed using the ImageStream high speed imaging cytometer. In the first system, pathogenic bacteria are distinguished from microflora and debris normally present in alfalfa seed sprouts. Because the aerobic conditions necessary for sprout growth also foster microbial growth, sprouts harbor a diverse microflora, including potential human pathogens. Salmonella spp., probed using a novel FISH method, are detected within complex sprout preparations by combining fluorescence/SSC intensity-based measurements with morphologic confirmation of gated populations. In the second study we measure length of individual bacteria for large populations of E.Coli and Cyanobacteria to follow bacterial growth in culture. By combining high speed image collection with quantitative imagebased measurements, the ImageStream enables accurate identification and classification of bacteria within heterogeneous samples.

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Related technologies: Imaging flow cytometry

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Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

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