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A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Spectral Instruments Imaging

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

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Detect structural variants at 1% allele fraction: Dive deeper into heterogeneous cancer samples

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To get a complete picture of highly rearranged cancer genomes in heterogeneous samples, use Bionano genome imaging...

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Insulin receptor substrate 1 is a substrate of the Pim protein kinases

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The goal of these studies was to identify Pim substrate(s) that could help define the pathway regulated by these...

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Webinar recording: Isolating ultra-high molecular weight (UHMW) DNA for genome analysis

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Identification and measurement of bacterial size using the ImageStream

Apr 27, 2016

In this note, we describe two bacterial studies performed using the ImageStream high speed imaging cytometer. In the first system, pathogenic bacteria are distinguished from microflora and debris normally present in alfalfa seed sprouts. Because the aerobic conditions necessary for sprout growth also foster microbial growth, sprouts harbor a diverse microflora, including potential human pathogens. Salmonella spp., probed using a novel FISH method, are detected within complex sprout preparations by combining fluorescence/SSC intensity-based measurements with morphologic confirmation of gated populations. In the second study we measure length of individual bacteria for large populations of E.Coli and Cyanobacteria to follow bacterial growth in culture. By combining high speed image collection with quantitative imagebased measurements, the ImageStream enables accurate identification and classification of bacteria within heterogeneous samples.

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Related technologies: Imaging flow cytometry

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Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

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Flow cytometer capable of high resoluition microscopic assays

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Combine the speed and statistical power of flow cytometry with microscopy

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