2D models, though cost effective, fail to replicate the 3D complexity of an in vivo tumor and associated influences of the tumor microenvironment, such as that of cell-to-cell contact and the influence of the extracellular matrix. These complex interactions, in combination with the unique properties of a 3D structure, affect tumor cell properties and behavior, gene expression, distribution of proteins, and ultimately, response to anticancer drugs. Spheroid models have a layered structure with rapidly proliferating cells surrounding a more quiescent and hypoxic, necrotic core. This structure generates a gradient of nutrients, metabolites, and oxygen in the spheroid, important attributes for evaluation of drug resistance due to penetration effects in a heterogeneous tumor. Effective analysis of 3D tumor spheroids can be challenging, yet models are emerging that can overcome those limitations and drive future research.
Learning objectives:
-Learn the two main approaches to generating 3D cancer models (liquid- and scaffold-based) and the limitations for assessing the growth and shrinkage of 3D tumor spheroids
-Discover how live-cell analysis can be combined with advanced cell models for added insight and productivity
-Glean what advances researchers can expect in the future
-Have the opportunity to ask questions during the live broadcast.
Keith L. Ligon, M.D., Ph.D., Brigham and Women’s Hospital, Boston, MA
Thursday, 16 May 2019, 6 PM Central Europe (CEST)
Register now!