Preparative electrophoresis systems for targeted size selection of biomolecules.
Automated microscopy and image analysis
Real-time, label free cell analysis
Nano and micro particle analysis
Accelerate to discover
Back to filter
Show all topics (10)
Related topics
Nov 30, 2021
Microfluidics have revolutionized...
Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 23, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
Nov 10, 2021
Join our webinar to learn how...
Discover the advanced Detector Technology in Agilent NovoCyte flow cytometers
Nov 8, 2021
This technical overview describes advancements in detector technology, and highlights examples where optimal signal...
Oct 20, 2021
Ionizing radiation approaches are effective but use of radioactive radioisotopic elements have become difficult to...
Duke uses XRAD 225 CX to test radiosensitivity hypothesis
Oct 18, 2021
Image guided radiotherapy in a preclinical setting couldn’t be more useful than the treatment of brain tumors, where...
Curiox webinar: Laminar Wash enables single-cell multiomics and cell hashing
Oct 11, 2021
Professor Mats Bemark’s lab at the University of Gothenburg in Sweden studies how the immune system responds to...
Falling in love with Magnetic resonance
Oct 4, 2021
Magnetic Resonance (MR) is more than just analytical science, it is unique, it is a passion that won’t let you go
Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping
Jul 20, 2018
Scientists from Wageningen University & Research and other institutions presented technical challenges of obtaining sufficient high molecular weight (HMW) nuclear DNA due to cell walls which are difficult to disrupt. The obtained nuclei are embedded into agarose plugs for processing and isolating uncontaminated HMW DNA, which is a prerequisite for nanochannel-based next-generation optical mapping strategies.
They described important improvements for obtaining HMW DNA that they tested on Solanum crops and wild relatives. Flow sorted nuclei were embedded in agarose plugs and HMW DNA was purified with proteinase K and RNAse A and then isolated. Pulsed-field gel electrophoresis (PFGE) was used for DNA quality control. HMW DNA was subsequently labeled and analyzed on the Irys platform. Optical mapping (OM) data were then processed for de novo OM and hybrid assemblies.
Related technologies: Targeted DNA/protein size collection
Get more info
Brand profile
