Accelerate to discover

Back to filter

Related topics

Cytek webinar - the new age of spectral flow cytometry

Cytek Biosciences

Sep 9, 2019

The Cytek Aurora flow cytometer has made more than twenty-four color flow cytometry panels possible with only three...

New study resolves helicase dynamics and interactions during DNA replication using C-Trap

LUMICKS

Aug 30, 2019

Researchers from Rockefeller University used a C-Trap optical tweezers – fluorescence microscopy system to observe the...

Low-dose maging in a new preclinical total-body PET/CT scanner

Bruker Biospin

Aug 28, 2019

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Novel methods to analyze platelet activation with the NovoCyte flow cytometer

Acea Biosciences

Aug 26, 2019

Platelets are of large importance in medicine both for their essential role in hemostasis and thrombosis, as well as...

Sep 16, 2019

Assessment of pro-arrhythmic effects using xCELLigence Cardio system and Pluricyte Cardiomyocytes

Acea Biosciences

Aug 21, 2019

The combination of Pluricyte Cardiomyocytes and the xCELLigence RTCA Cardio ECR system enables  detailed ...

Bruker announces acquisition of preclinical and molecular imaging research software provider PMOD

Bruker Biospin

Aug 19, 2019

The PMOD software is widely used for the analysis of positron emission tomography (PET) studies in neurology,...

Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

Sage Science

Aug 16, 2019

The BluePippin was used for High Pass DNA size selection prior to library construction. ONT Promethion was used for...

Sep 16, 2019

Fluxion Biosciences launches custom liquid biopsy NGS panel development

Fluxion Biosciences

Aug 14, 2019

Fluxion a leading developer of precision solutions for life science research and diagnostics, announced that can...

Show all topics (10)

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping

Jul 20, 2018

Scientists from Wageningen University & Research and other institutions presented technical challenges of obtaining sufficient high molecular weight (HMW) nuclear DNA due to cell walls which are difficult to disrupt. The obtained nuclei are embedded into agarose plugs for processing and isolating uncontaminated HMW DNA, which is a prerequisite for nanochannel-based next-generation optical mapping strategies.
They described important improvements for obtaining HMW DNA that they tested on Solanum crops and wild relatives. Flow sorted nuclei were embedded in agarose plugs and HMW DNA was purified with proteinase K and RNAse A and then isolated. Pulsed-field gel electrophoresis (PFGE) was used for DNA quality control. HMW DNA was subsequently labeled and analyzed on the Irys platform. Optical mapping (OM) data were then processed for de novo OM and hybrid assemblies.

Scientific paper
Application

Related technologies: Targeted DNA/protein size collection

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

Related products

Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

show detail

Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

show detail

Automated size selection for 24 samples at a time.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey