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Modeling multiple myeloma-Bone Marrow interactions using Synthecon rotating-wall vessels

Dec 2, 2020

To this purpose, scientists exploited the Synthecon 3D Rotary Cell Culture System bioreactor technology for myeloma-bone marrow co-cultures in gelatin scaffolds. The model was validated with myeloma cell lines that, as assessed by histochemical and electron-microscopic analyses, engaged contacts with stromal cells and endothelial cells. Consistently, pro-survival signaling and also cell adhesion-mediated drug resistance were significantly higher in 3D than in 2D parallel co-cultures. The contribution of the VLA-4/VCAM1 pathway to resistance to bortezomib was modeled by the use of VCAM1 transfectants. Soluble factor-mediated drug resistance could be also demonstrated in both 2D and 3D co-cultures. The system was then successfully applied to co-cultures of primary myeloma cells-primary myeloma bone marrow stromal cells from patients and endothelial cells, allowing the development of functional myeloma-stroma interactions and MM cell long-term survival. Significantly, genomic analysis performed in a high-risk myeloma patient demonstrated that culture in bioreactor paralleled the expansion of the clone that ultimately dominated in vivo. Finally, the impact of bortezomib on myeloma cells and on specialized functions of the microenvironment could be evaluated. Our findings indicate that 3D dynamic culture of reconstructed human multiple myeloma microenvironments in bioreactor may represent a useful platform for drug testing and for studying tumor-stroma molecular interactions.

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Synthecon

Inovating 3D cell culture technology exploring the rotary culture system for tissue engineering, stem cell, tumor spheroid and more. Unique Rotary Cell Culture System (RCCS)  for 3D culturing both suspension and anchorage-dependent cells.

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The disposable (single use) vessel culture systems are low cost, easy to use, rotary bioreactor systems that utilize disposable culture vessels to enable 3D cell culture.

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The autoclavable RCCS use culture vessels designed for repeated autoclaving, and ideal for long-term use. Suitable for any of the Synthecon culture vessels supplied with variable speed motor drives equipped with tachometer.

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The RCCS-SC systems are a unique 3D cell culture technology with scaled down size for economic and more efficient stem cells growth.

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RCCMAX allows undisturbed 3D cultivation of cells in vitro condition with continuous supplementation of fresh media and oxygen.

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