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Monitoring Lung tumor using Bioluminescence, Fluorescence and microCT

Apr 14, 2020

In this study, the relative synergies of in vivo BLI, Fluorescence Imaging (FLI), and micro-CT imaging was assessed in a classic orthotopic lung cancer model from onset to late stage progression. The tumor progression study was performed using a dual bioluminescent/fluorescent reporter human lung carcinoma cell line (A549-FLuc-Neo/iRFP-Puro; Imanis Life Sciences, Rochester, MN, USA) in athymic nude female mice (Envigo RMS, Inc. Indianapolis, IN, USA). Pulmonary tumor foci were initiated by i.v. tail vein injection. Imaging was performed weekly to assess limits of detection and resolution for each modality. Ex vivo imaging was also performed. Optical imaging was performed using the In-Vivo Xtreme II optical/X-ray imaging system (Bruker BioSpin, Billerica, MA, USA), and microCT imaging was completed using the SkyScan 1176 & 1272 scanners (Bruker microCT, Kontich, Belgium).

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