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New protocol: Analyze small extracellular RNAs with PippinHT

May 18, 2018

If your research involves working with small RNAs, be sure to check out this carefully documented protocol published in Extracellular RNA, part of Springer Nature’s Methods in Molecular Biology book series. Authors present an NGS protocol designed for use with low-input biofluid samples containing extracellular RNAs. The protocol “has modifications designed to reduce the sequence-specific bias typically encountered with commercial small RNA library construction kits,” the authors report. The result is increased library diversity and more complete RNA profiles. As the writers note, extracellular RNA has great promise for use as a biomarker or diagnostic, offering a non-invasive approach to everything from monitoring cancer to prenatal testing. The challenge for scientists, though, is that typical small RNA library prep kits require higher concentrations than can be gleaned from biofluid samples such as plasma or serum. This new protocol optimizes steps for samples that do not meet those concentration thresholds.

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Related technologies: Targeted DNA/protein size collection

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Preparative electrophoresis systems for targeted size selection of biomolecules.

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Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

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Automated size selection for 24 samples at a time.

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