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New study resolves helicase dynamics and interactions during DNA replication using C-Trap

Aug 30, 2019

Wasserman et al. analyzed the properties of fluorescently-labeled CMG during DNA replication. For this, they used optical tweezers to tether a double-stranded molecule between two beads and create DNA forks in situ by force manipulation. They then visualized the behavior of single CMG complexes using fluorescence imaging while subjecting the replisome assembly to different reaction conditions by utilizing the laminar flow microfluidics.

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Optical tweezers, Confocal microscopy, STED nanoscopy, Acoustic force spectroscopy for single-molecule biophysics. 

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Live, simultaneous and correlative visualization and manipulation of molecular interactions with sub-picoNewton force resolution and sub-nm position resolution.

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The first dedicated optical tweezers instrument specifically developed for high-resolution single-molecule force spectroscopy research.

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Acoustic Force Spectroscopy is a single-molecule, lab-on-a-chip technology in which acoustic waves are used to exert forces on thousands of biomolecules.

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