Preparative electrophoresis systems for targeted size selection of biomolecules.
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PacBio-LITS in combination with Sage Science automated size selection
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The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. With SDS-agarose gel cassettes, the BluePippin collects targeted protein sizes from complex samples for biomarker or protein modification studies. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.
In this BMC Genomics publication, scientists from Baylor College of Medicine report a new method for targeted sequencing of important structural variations in the human genome with the PacBio platform. Using oligo-based DNA capture with Pippin Prep or BluePippin sizing from Sage Science, the researchers demonstrated successful structural variant analysis at high accuracy and low cost.
Related technologies: Targeted DNA/protein size collection
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