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High Pass size selection removes smaller DNA fragments from a sheared genomic DNA (or sequencing library) while collecting the remaining larger fragments above a tightly controlled size threshold. This way, larger molecules can be presented to the detector or droplet, and better sequencing performance can be achieved. The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes). We’ve also increased the size of the elution module and surface area of the filtration membrane, bringing about improved sample recovery and reproducibility.
Related technologies: Targeted DNA/protein size collection
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Preparative electrophoresis systems for targeted size selection of biomolecules.
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www.sagescience.com/
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