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Real-time chemotaxis of macrophages

Jul 15, 2016

Iqbal and colleagues (PLoS ONE. 2013 Mar; 8(3): e58744) used the xCELLigence RTCA DP system and CIM-Plate to quantify the migration of murine macrophages in response to CC chemokines and other chemoattractant signaling molecules.They stated that “the xCELLigence real-time migration assay offers a rapid and reproducible system to monitor macrophage chemotaxis,” and “quantification is also far more rapid and accurate compared to the modified Boyden chamber where multiple images are required followed by staining and counting which can also introduce operator bias”.

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Related technologies: Real-time, Label Free Cell Analysis

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Riccardo Pasculli

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Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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A unique, portable, impedance-based system for cell-based assays, such as cell growth, proliferation, cytotoxicity, adhesion and morphological dynamics

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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Cardio Instrument is a high-resolution system for label-free-dual-mode monitoring of cardiomyocyte and cardiotoxicity testing

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The new CardioECR system combines impedance and Multi Electrode Array (MEA) technology with a pacing function

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