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STAT Protein Phosphorylation in CD4+ T Cells using the CellStream flow cytometer

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Real-time, dynamic measurements of cellular migration & controlled microenvironmental conditions

Sep 13, 2017

Typically, cellular migration is studied using a two compartment device called a Boyden chamber or a transwell insert system. The data gathered using a Boyden chamber are narrowly-focused snapshots into complex biological phenomena. Events occurring before the endpoint are typically lost using the Boyden chamber or transwell approach. The processing of transwell inserts for data capture is considerably labor-intensive. Further, it is impossible to control environmental conditions within the well due to the static nature of the assay. In order to examine these processes in detail over time, it is necessary to observe cells in situ.

BioFlux allows observation of chemotaxis and migration as a dynamic event:

- Up to 16 conditions tested in parallel

- Flexible format (liquid or gel) enables a wider range of assays

- Better quality data with real-time measurements in situ

- Minimizes variability with a continuous chemotactic gradient

- Saves time by collecting data direct from the assay with no transfer steps

- Assays can be performed label-free

 

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Related technologies: Cell interaction studies

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Riccardo Pasculli

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Riccardo

Pasculli

+420 731 127 717

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Fluxion Biosciences

With BioFlux System for live cell analysis under shear flow and IsoFlux System for circulating tumor cell analysis, Fluxion Biosciences turn power of benchtop cellular analysis with advanced tools to automate complex assays.

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System that offers a fully-integrated solution for automated, high throughput shear flow assays

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Core control system, integrated to any inverted microscope to introduce flow to your research

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