Accelerate to discover

Back to filter

Related topics

Imaging Flow Cytometry Luminex learning sessions

Luminex

Mar 5, 2021

Start the new year with new knowledge! Get the most from your Amnis instrument and IDEAS Software with training...

Preclinical lung imaging offers effective evaluation of drug candidates

Bruker Biospin

Mar 1, 2021

The incidence of respiratory disease is increasing throughout the world, and the demand for safe, effective drugs to...

Online courses on Stroke, Diabetes, VCID research

RWD

Feb 18, 2021

Are you interested in research of Stroke, Diabetes, or VCID Research? Do you want to share your knowledge with other...

Curiox Laminar Wash technology to become a gold standard for TotalSeq Protocol.

Curiox

Feb 15, 2021

Curiox Laminar Wash technology has been added as part of the official BioLegend TotalSeq Protocol. It makes processing...

Mar 8, 2021

Amnis CellStream's new software features - now better than ever

Luminex

Feb 12, 2021

It's finally here, and better than ever - Amnis CellStream's Acquisition and Analysis Software update increases ease of...

The function of LSCI with off Line Model

RWD

Feb 10, 2021

In this video, we will show you how to change the pseudo-color off-line. At the same time, we will show you how to...

Towards silent and distortion-free fMRI with Zero Echo Time MRI

Bruker Biospin

Feb 9, 2021

By using fast imaging techniques capable of scanning large brain regions within short durations, continuous snapshots...

Mar 8, 2021

Cytek Aurora in Action : how to easily resolve highly overlapping dyes ?

Cytek Biosciences

Feb 2, 2021

Learn from Jeff Nelson, a manager of a cytometry shared resource laboratory, about the Cytek Aurora's unique features...

Show all topics (10)

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Jan 17, 2019

The development of effective bioanalytical methods for rapid, sensitive and specific detection of HOCl in vitro and in vivo plays a key role for better understanding the roles of this molecule in normal and diseased conditions, but remains challenging due to the highly reactive nature of HOCl and the complicated biological conditions. In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. PQI was easily synthesized by a one‐step condensation reaction. Upon addition of HOCl, significant changes in the absorption spectra and the color of the solution were noticed, facilitating the “naked eye” detection of HOCl in PBS buffer. The fluorescence of PQI was found to be significantly increased within a few seconds, leading to “OFF‐ON” fluorescence response towards HOCl. The sensing mechanism, oxidation of thioether by HOCl, was confirmed by HRMS titration analysis. PQI features a large Stokes shift, high sensitivity and selectivity, and rapid fluorescence response towards HOCl. Quantitative detection of HOCl in single live cells was demonstrated through fluorescence imaging and flow cytometry analysis. PQI was then successfully used in visualisation of HOCl in live zebrafish and nude mice.

Do you want to learn more? Read this interesting article!

Scientific paper
Application

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 255 700 886

Send Message

Brand profile

Spectral Instruments Imaging

Spectral Instruments Imaging manufactures instruments for preclinical optical (bioluminescent, fluorescent) and X-ray imaging.

Related products

System for individual researchers and smaller specialist teams to conduct optical imaging studies in disease model progression, response to therapy, and cell migration in-vivo.

show detail

The Ami HT and Ami HTX (includes X-Ray imaging) optical imaging systems establish a new, high throughput benchmark for in vivo imaging suitable for specialist researchers and small teams.

show detail

The Lago and X‐Ray capable Lago X optical imaging systems provide a powerful and flexible in-vivo imaging capability suitable for imaging cores, specialist researchers and small teams, delivering an unmatched 10 mouse capacity across BLI, FLI and X‐Ray.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey