Accelerate to discover

Back to filter

Related topics

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

ACCELA educational webinar: Unlocking Spatial Biology with RareCyte Orion

RareCyte

Mar 16, 2023

Experience Spatial Biology with Orion Multiplex Imaging. Orion is a benchtop, high...

Recorded webinar: Anesthesia Considerations in Small Animal Imaging

Spectral Instruments Imaging

Mar 13, 2023

Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...

Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay

Cytek Biosciences

Mar 13, 2023

This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...

Mar 25, 2023

Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II

RareCyte

Mar 7, 2023

CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...

NanoCellect Webinar: Optimizing the Cell Line Development Process with Microfluidic Cell Sorting

NanoCellect

Mar 3, 2023

Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...

New Kit for Bacterial analysis expands capability of Guava Muse flow cytometer

Luminex

Feb 20, 2023

With the Guava Muse cell analyzer, you can now achieve highly quantitative results at a fraction of the price, effort,...

Mar 25, 2023

Flash news - did you know, that you can use Singulator S100 also for plant research?

S2 Genomics

Feb 10, 2023

In this short TechNote you can find how Singulator can help the data quality in Single cell sequencing - now even in...

Show all topics (10)

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Jan 17, 2019

The development of effective bioanalytical methods for rapid, sensitive and specific detection of HOCl in vitro and in vivo plays a key role for better understanding the roles of this molecule in normal and diseased conditions, but remains challenging due to the highly reactive nature of HOCl and the complicated biological conditions. In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. PQI was easily synthesized by a one‐step condensation reaction. Upon addition of HOCl, significant changes in the absorption spectra and the color of the solution were noticed, facilitating the “naked eye” detection of HOCl in PBS buffer. The fluorescence of PQI was found to be significantly increased within a few seconds, leading to “OFF‐ON” fluorescence response towards HOCl. The sensing mechanism, oxidation of thioether by HOCl, was confirmed by HRMS titration analysis. PQI features a large Stokes shift, high sensitivity and selectivity, and rapid fluorescence response towards HOCl. Quantitative detection of HOCl in single live cells was demonstrated through fluorescence imaging and flow cytometry analysis. PQI was then successfully used in visualisation of HOCl in live zebrafish and nude mice.

Do you want to learn more? Read this interesting article!

Scientific paper
Application

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 210 323 421

Send Message

Brand profile

Spectral Instruments Imaging

Spectral Instruments Imaging manufactures instruments for preclinical optical (bioluminescent, fluorescent) and X-ray imaging.

Related products

System for individual researchers and smaller specialist teams to conduct optical imaging studies in disease model progression, response to therapy, and cell migration in-vivo.

show detail

The Ami HT and Ami HTX (includes X-Ray imaging) optical imaging systems establish a new, high throughput benchmark for in vivo imaging suitable for specialist researchers and small teams.

show detail

The Lago and X‐Ray capable Lago X optical imaging systems provide a powerful and flexible in-vivo imaging capability suitable for imaging cores, specialist researchers and small teams, delivering an unmatched 10 mouse capacity across BLI, FLI and X‐Ray.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey