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Small animal 13C-hyperpolarized MRI

Bruker Biospin

Feb 8, 2019

13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

Spectral Instruments Imaging

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Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Spectral Instruments Imaging

Jan 17, 2019

In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. The...

Improved differentiation of Retinal Organoids stem cells derived using rotating-wall vessels

Synthecon

Jan 15, 2019

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a...

Feb 20, 2019

CT‐analyzer plug‐ins: ROI shrink‐wrap and primitive ROI

Bruker Biospin

Jan 7, 2019

The ROI shrink‐wrap gives the possibility to automatically generate a region‐of‐interest by adapting to the shape of...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

Dec 17, 2018

MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

Feb 20, 2019

Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies

Etaluma

Dec 6, 2018

Accurate labeling of endogenous proteins for advanced light microscopy in living cells remains challenging. Nanobodies...

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Single-cell measurement of the uptake, intratumoral distribution using Helios mass cytometer

Mar 22, 2016

We used the recently-developed technique of mass cytometry to measure cisplatin uptake into individual tumor cells (Pt atoms/cell), combined with measurement of the rate of IdU incorporation into DNA (I(127) atoms/cell/min) and tumor hypoxia identified by the 2-nitroimidazole EF5 in cisplatin-treated BxPC-3 and ME-180 xenografts. Pt levels of 10(5) to 10(6) atoms/cell were obtained following a single cisplatin treatment using clinically relevant doses. Cisplatin caused cell cycle arrest in a dose- and time-dependent manner that paralleled effects in vitro, and it readily penetrated into hypoxic tumor regions. Similar levels of Pt/cell were found in xenografts treated with oxaliplatin. Mass cytometry offers the unique capability to study the cellular uptake and anticancer effects of platinum-containing drugs at the single cell level in animal models, and it has the potential for application to samples obtained from cancer patients during treatment.

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Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

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