Curiox Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry, eliminating the usage of the centrifuge.
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Single Cell Sequencing sample preparation without centrifugation
Mar 10, 2020
Single cell sequencing can suffer from poor data quality due to clumping of cells, incomplete washing of cells leaving cell-free nucleic acids, and poor retention of low numbers of cells. Washing via centrifugation causes stress on the cells in samples and can cause erroneous gene expression results. Laminar Wash systems can wash cells without putting stress on cells that may impact gene expression, cell integrity and viability. Your fellow researchers using single-cell applications have been engaging us and the Laminar Wash technology for their sample prep needs.
Benefits include:
• No need to spin cells to concentrate them
• Reduction of cell debris
• Minimize cell clumping
Centrifuged samples have inconsistent retention, with examples of large clumps. Nuclei are more distinct when washed on Laminar Wash systems. Retention numbers with Laminar Wash systems are higher and more consistent.
Related technologies: Automated sample preparation
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