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Single Cell Sequencing sample preparation without centrifugation

Mar 10, 2020

Single cell sequencing can suffer from poor data quality due to clumping of cells, incomplete washing of cells leaving cell-free nucleic acids, and poor retention of low numbers of cells. Washing via centrifugation causes stress on the cells in samples and can cause erroneous gene expression results. Laminar Wash systems can wash cells without putting stress on cells that may impact gene expression, cell integrity and viability. Your fellow researchers using single-cell applications have been engaging us and the Laminar Wash technology for their sample prep needs.

Benefits include:
• No need to spin cells to concentrate them
• Reduction of cell debris
• Minimize cell clumping

Centrifuged samples have inconsistent retention, with examples of large clumps. Nuclei are more distinct when washed on Laminar Wash systems. Retention numbers with Laminar Wash systems are higher and more consistent.

Learn more on Curiox application page!

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Related technologies: Automated sample preparation

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Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

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Curiox

Curiox Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry, eliminating the usage of the centrifuge.

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An affordable, benchtop instrument for flow cytometry sample preparation

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Superior consistency and robust flow cytometry data using centrifuge-less washing

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