Accelerate to discover

Homepage>Discover>Squaraine fluorophore as a NIR-fluorescent probe for the in vivo detection of diagnostic enzymes

Jul 1, 2020

Get answers to your questions on 3D spheroid-based drug discovery, organoid research and working with advanced cell...

Bruker MicroCT systems supporting suality control for refurbished FFP masks

Jun 29, 2020

In the effort to tackle the coronavirus pandemic, Bruker is proud that its instruments are being used to ensure quality...

Advance your Neuro-Oncology applications with IncuCyte

Jun 25, 2020

Neuro-oncology research can benefit from robust translational in vitro models to gain greater understanding of brain...

MicroCT Applications in Lung research and Immunology

Jun 22, 2020

The ability to monitore and quantitate lung disease in real time is an advantage to using small animal microCT. Also,...

Jul 3, 2020

Imaging Extracellular Vescicles with super-resolution microscopy

Jun 18, 2020

Extracellular vesicles (EVs) play key roles in cell-to-cell communication. EVs can cross biological barriers (such as...

The Role of Micro-CT in Imaging Breast Cancer Specimens

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

Jul 3, 2020

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

Show all topics (10)

Squaraine fluorophore as a NIR-fluorescent probe for the in vivo detection of diagnostic enzymes

Nov 19, 2018

Squaraine dyes have excellent photostability with intensive absorption and strong fluorescence in the near-infrared (NIR) region. However, they display a strong tendency to aggregate in aqueous media because of their poor water solubility, often causing fluorescence quenching that severely limits their in vivo applications, especially for detecting or imaging diagnostic enzymes. In this work, an oligo(ethylene glycol)-functionalized squaraine fluorophore has been developed as an NIR-fluorescent probe that can detect and image the activities of a diagnostic enzyme (leucine aminopeptidase) both in vitro and in vivo. The probe shows near-infrared absorption and emission, a low detection limit (0.61 ng/mL), relatively good aqueous solubility, high selectivity, and little toxicity. In addition, the probe herein was successfully used to track endogenous leucine aminopeptidase both in vitro and in vivo with a nude-mouse model.

Do you want to learn more? Read this interesting article!

Scientific paper

Application

Get more info