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To ensure detection of low abundance mtDNA mutations without interference from nuclear pseudogenes, many researchers enrich mitochondria using differential gradient centrifugation, or enrich specific mitochondrial DNA sequences by PCR, before NGS sequence analysis. Scientist demonstrate a novel approach to mtDNA isolation using the SageHLS platform which provides enrichment levels comparable to gradient centrifugation, but with a small fraction of the hands-on time.
Related technologies: Targeted DNA/protein size collection
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Preparative electrophoresis systems for targeted size selection of biomolecules.
More info at:
www.sagescience.com/
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