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Targeted mitochondrial DNA extraction and enrichment using the SageHLS

Oct 31, 2019

To ensure detection of low abundance mtDNA mutations without interference from nuclear pseudogenes, many researchers enrich mitochondria using differential gradient centrifugation, or enrich specific mitochondrial DNA sequences by PCR, before NGS sequence analysis. Scientist demonstrate a novel approach to mtDNA isolation using the SageHLS platform which provides enrichment levels comparable to gradient centrifugation, but with a small fraction of the hands-on time.

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Related technologies: Targeted DNA/protein size collection

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Regina Fillerová

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Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

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Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

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Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

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Automated size selection for 24 samples at a time.

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