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Targeted sequencing of highly homologous genes using Cas9 digestion

Nov 25, 2019

Sage Science has developed an instrument that can accept isolated white blood cells as the input sample and perform DNA extraction, Cas9 digestion, and preparative target purification in an integrated, semi-automated fashion. PKD1 gene, which implicated in polycystic kidney disease, has homology with six closely related pseudogenes. PKD1 was isolated with the SageHLS system and sequenced with the Illumina platform.