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Timelapse analysis of cell migration using CQ1 confocal quantitative image cytometer

Sep 6, 2017

The scratch was created on the cultures of Fucci-HeLa cells. Mitomycin C (MMC) was added to the culture medium to interrupt the cell-division cycle then washed out. Time-lapse imaging was performed for three days.The scratch was infiltrated by the cells in G1 and S/G2/M phase in the control cultures, indicating that the scratch was infiltrated not only by cell migration but also by proliferation as well. On the other hand, most of the infiltrating cells were in S/G2/M phase in the MMC-treated culture, indicating that the scratch was filled mostly by cell migration.

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Riccardo

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