Use of the real time xCelligence system for purposes of medical microbiology

Celligence impedance-measuring instrument is one of a few commercially available systems of such type. The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, measurement of bacterial biofilm formation and impact of antiseptics on the biofilm structure. To test the infuence of bacterial enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary (CHO) cell line and reference strain Escherichia coli ATTC 35401 were used. To investigate instrument ability to measure biofilm formation and impact of antiseptics on its development, Staphylococcus aureus ATTC6538 reference strain was used. The data generated during the experiments indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin and to detect staphylococcal biofilm formation.  xCelligence instrument can be used for real-time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real-time measurement of staphylococcal biofilm formation in vitro.

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