ACEA Biosciences developed a label-free assay format that allows dynamic monitoring of NK cell-mediated cytolysis. The assay uses impedancebased technology (RT-CES)4,5. This system is the predecessor of the new xCELLigence System. The attachment and interaction of adherent cells with wells on a 96X E-Plate leads to impedance changes, which correlate with cell number, size and shape. In contrast, addition of suspended cells to the wells results in undetectable changes due to non-existent or weak interaction with the electrodes. Therefore, NK cell-mediated cytolytic effects on the cancer cell monolayer can be quantitatively and dynamically monitored on the sensor plate without labeling the target cells.
Read more