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HNPCC MASTR Plus - Multiplicom

Mutations and CNVs in the MLH1, MSH2, MSH6 and PMS2 genes

The HNPCC MASTR Plus is a molecular assay (research use only) for the identification of all mutations and CNVs in 4 genes (MLH1, MSH2, MSH6, and PMS2) and 3’ UTR of EPCAM associated with hereditary non-polyposis colorectal cancer (HNPCC). This assay is provided as a ready-to-use kit that offers robust performance with minimum hands-on time. All reagents necessary to enable multiplex amplification of 84 amplicons (277-443 bp) in 5 PCR reactions are included, for complete coverage of all coding sequences and selected intronic regions. The assay is compatible with all current Massively Parallel Sequencing (MPS) systems, providing the flexibility to choose your preferred method. Hereditary Non-Polyposis Colorectal Cancer syndrome, also called, Lynch syndrome, is an inherited tendency to develop colorectal, endometrial (uterine) and other cancers that occur typically in the fourth decade of life. About 5% of people who have colorectal or endometrial cancer develop Lynch syndrome.  The HNPCC genes are called “mismatch repair genes” and their main function consists of repairing DNA of incorrectly paired bases during DNA replication.  When a mutation occurs in one of those mismatch repair genes, DNA is not repaired properly and predisposes all of the body’s cells to cancer.

Current methods, such as Sanger-based sequencing, are time consuming, labor-intensive and expensive. Identification of mutations by MPS provides a faster and cost-effective approach.

Note: This assay is to be used for research use only.

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http://www.multiplicom.com/product/hnpcc-mastr-plus-0
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