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SOMATIC 2 MASTR Plus - Multiplicom

Mutations in the EGFR, ERBB2, PIK3CA, PIKR1, and PTEN genes from FFPE-derived DNA

The SOMATIC 2 MASTR Plus is a molecular assay for the identification of all mutations in the coding region of the EGFR, ERBB2, PIK3CA, PIK3R1 and PTEN genes in various types of solid tumors. Multiplicom’s SOMATIC 2 MASTR Plus assay is provided as a ready-to-use kit that offers robust performance with minimum hands-on time. All reagents necessary to enable multiplex amplification of 146 amplicons (124-237 bp) in three PCR reactions are included, for complete coverage of all coding sequences.

The EGFR, ERBB2, PIK3CA, PIK3R1 and PTEN genes are involved in the PTEN/PI3K/AKT/mTOR pathway that regulates signaling of multiple biological processes such as metabolism, cell proliferation, cell growth and apoptosis. Inappropriate activation of the PI3K pathway that signals downstream from receptor tyrosine kinases is frequently activated in many cancers.

Somatic mutations in the EGFR, ERBB2, PIK3CA, PIK3R1 and PTEN genes are observed in different tumor tissues. These mutations are associated with overexpression of encoding protein, enhanced lipid kinase activity, protein truncation, activation of AKT, and TOR-mediated signaling and oncogenicity.  Therefore, the use of a single test, such as the SOMATIC 2 MASTR Plus, provides an excellent tool for simultaneously and accurate identification of a broad spectrum of genomic alterations in the biological pathways from a single biopsy. 

Current methods, such as Sanger-based sequencing, are time consuming, labor-intensive, expensive and not sensitive for detection of low frequency mutations. Identification of mutations by MPS provides a faster and cost-effective approach.

Note: This assay is to be used for research use only.

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http://www.multiplicom.com/product/somatic-2-mastr-plus
Petra Kupková

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