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"We plan to use the Pippin prep for our NGS library purification."

Dr. Jiri Baloun

CEITEC – Central European Institute of Technology, Brno, Czech Republic

BluePippin - Sage Science

Targeted Size Selection for Next-Gen Sequencing

The Pippin Systems facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The current high-throughput DNA sequencers — including platforms from Illumina, Ion Torrent, and 454 - require tightly sized insert libraries for optimum sequencing performance. The platform features the ability to collect narrow and even fragment distributions, without contamination. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer.

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.  With SDS-agarose gel cassettes, the BluePippin collects targeted protein sizes from complex samples for biomarker or protein modification studies. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.

BluePippin automatically collects DNA fragments in range from 90 bp -50kb.

Benefits of the BluePippin System

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies

  • Mate-pair sequencing
  • Single molecule sequencing
  • Protein mass spec

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http://www.sagescience.com/products/bluepippin/
Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

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