"We plan to use the Pippin prep for our NGS library purification."

Dr. Jiri Baloun

CEITEC – Central European Institute of Technology, Brno, Czech Republic

"Blue Pippin is great system for automated electrophoresis, which allows us to collect fragments with lenght over 10k bp.The system is very precise and easy to use."

Dr. Jaroslav Hrabák

Biomedical Center, Pilsen, Czech Republic

BluePippin - Sage Science

Targeted Size Selection for Next-Gen Sequencing

The Pippin Systems facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The current high-throughput DNA sequencers — including platforms from Illumina, Ion Torrent, and 454 - require tightly sized insert libraries for optimum sequencing performance. The platform features the ability to collect narrow and even fragment distributions, without contamination. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer.

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.  With SDS-agarose gel cassettes, the BluePippin collects targeted protein sizes from complex samples for biomarker or protein modification studies. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.

BluePippin automatically collects DNA fragments in range from 90 bp -50kb.

Benefits of the BluePippin System

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies

  • Mate-pair sequencing
  • Single molecule sequencing
  • Protein mass spec

Show more

Discover more

Method to simultaneously construct 12 differently sized Illumina Nextera long mate pair libraries PacBio-LITS in combination with Sage Science automated size selection Targeted size selection for next-gen sequencing (100bp – 1.5kb) Sage Science launches instrument to purify high-quality DNA directly from cells Size selection is essential for generating excellent results Size selection of cDNA fragments with PippinHT Long-read sequencing of coffee bean transcriptome reveals the diversity of full-length transcripts Mapping and phasing of structural variation in patient genomes using nanopore sequencing Target large genomic regions with 10x genomics using SageHLS New protocol: Analyze small extracellular RNAs with PippinHT Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping BluePippin-DNA size selection for NGS with pulsed-field (100bp-50kb) Target large genomic regions with 10x genomics + SageHLS Product update: The high pass plus cassette HLS-CATCH: Cas-9 assisted gene purification Sage products powering new methods, 2018-19 Linked-read sequencing advances understanding of cancers Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome Targeted mitochondrial DNA extraction and enrichment using the SageHLS Targeted sequencing of highly homologous genes using Cas9 digestion Unleash Your Illumina Sequencer with TELL-Seq Linked Reads See What's Possible with the PippinHT An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing
Regina Fillerová

Field application specialist



+420 731 127 718

Send Message

Related products

Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

show detail

Pulsed-field Power for Gel Boxes

show detail

Automated size selection for 24 samples at a time.

show detail

Whole Sample Fractionation of DNA or Protein - Collect 12 Contiguous Fractions from a Single Sample

show detail

The automated platform to work with high molecular weight DNA for applications ranging from long-read sequencing to optical mapping.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic