Target Enrichment for NGS

Next-Generation Sequencing (NGS) technologies have transformed life science research, enabling many new sequencing-based applications. One of the most promising is targeted sequencing, to identify variations relevant to cancer, disease research, and population genetics. Using NGS as the foundation for a targeted sequencing approach promises to provide quantitative insight into mutations, in context, in a single run, at greater throughput, and at lower cost than previously seen.

The Access Array System or SmartChip Nano-Well Technology, combined with next-generation sequencing, is a powerful approach for comprehensive genotyping, validation, and screening analyses, including SNP identification, sequence variation and mutation detection, DNA methylation mapping, and exon sequencing—across hundreds of samples.

Library preparation is the foundation for a successful targeted sequencing run. However, traditional methods create bottlenecks and introduce risk to quality and reproducibility through the high number of steps and touch points in the protocols. The Access Array System, through its system of controllers and integrated fluidic circuits (IFCs), and SmartChip Nano-Well Technology encapsulate library preparation to provide unparalleled quality amplicons. These systems take DNA from sample to sequence-ready.