Accelerate to discover

Back to filter

Related topics

IncuCyte Live-Cell Analysis System - nearly 3,000 publications and counting!

Sartorius

Jan 21, 2020

IncuCyte has now reached over 2,000 cited publications! Search our publications filter to see what exciting research is...

Development of superparamagnetic nanoparticles as an efficient contrast agent for multimodal imaging

Bruker Biospin

Jan 16, 2020

Gadolinium‐based contrast agents are used to provide diagnostic information in clinical magnetic resonance...

IncuCyte live-cell analysis system for live-cell visualization of neuronal activity

Sartorius

Jan 13, 2020

A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and...

Latest updates to Bionano Access and Bionano Solve

BioNano Genomics

Jan 8, 2020

Bionano Access v1.5 and Bionano Solve v3.5 are the latest updates to Bionano visualization software and analysis tools....

Jan 22, 2020

The qNano Gold: Obtain the most detailed Information for each individual nanoparticle

IZON

Jan 6, 2020

The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful...

The importance of titrating Viability dyes on the Aurora Spectral Cytometer

Cytek Biosciences

Jan 2, 2020

What is the best way to decide when your viability is titrated properly? Unlike titrating antibodies how to determine...

New analysis open modules expand capabilities of Guava Muse flow cytometer

Luminex

Dec 30, 2019

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava Muse cell analyzer, you can now...

Jan 22, 2020

High molecular weight DNA from bone marrow aspirates samples in 4 hrs

BioNano Genomics

Dec 23, 2019

With Bionano Prep SP BMA DNA Isolation kit, transform the way you are isolating UHMW DNA from this complex sample type...

Show all topics (10)

Analysing “tissue sociology” with StrataQuest software

Feb 23, 2016

Cell in tissue plays a defined role as being a member of a small cell subunit within a larger organic structure. The development and differentiation of a given cell is defined by the parent cell and the local cell neighbourhood and environment. Consequently, the analysis of cells in their local environment is of major importance. StrataQuest analysis software contains specific image processing tools for the characterization of the social situation of cells in tissue. Apart from distance measurements there are tools to identify relationships of specific cells by selecting all cells in a local neighbourhood or functional group as a separate population.

The depicted skin sample was stained using the Multi-epitope Ligand Cartography (MELC) method (Fig. A). The tissue sample is subjected to repeated cycles of fluorescent staining, imaging and photo bleaching. Each cycle can use another antibody for a different antigen. The result is a set of images showing the distribution of each antigen in a different layer for the same sample.

The job for StrataQuest in this skin sample was to detect all cells and Melan-A positive areas (Fig. B). Based on this location information, the increase or decrease of the 90 antigen intensities in the areas vicinity was quantified . On this behalf, the Melan-A positive areas were automatically detected and distance transformation masks were established around each of them (Fig.C). The epidermis was also automatically detected (Fig. D).

StrataQuest has the capability to perform these automatic detection, measurement and analysis steps for any functional group of cells in such samples.

Application
Product news

Brand profile

TissueGnostics

TissueGnostics develops systems for automated identification and functional characterization of single cells in tissue sections, cultured and smeared cells as well as TMA. This includes automated image acquisition and image processing for immunohistochemistry, multi-chanel immunofluorescence and hematology.

Related products

Tissue analysis system for immunohistochemistry and immunofluorescence. TissueFAXSi Plus draws together features of HistoFAXS and TissueFAXS systems.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey