Accelerate to discover

Back to filter

Related topics

Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs

Fluxion Biosciences

Oct 12, 2018

Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...

Target large genomic regions with 10x genomics + SageHLS

Sage Science

Oct 9, 2018

With customized Cas9 nucleases, scientists can now isolate specific large genomic DNA fragments with the SageHLS...

Coy anaerobic chambers #1 choice in microbiology

COY laboratory products

Oct 5, 2018

Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

Understanding subtle cardiac changes

VisualSonics FujiFilm

Oct 3, 2018

Are you working with high ultrasound imaging and are you interesting in VevoStrain application and specific changes in...

Oct 15, 2018

EasyTEL+ unique digital platform for single or group-housed subjects e

emka TECHNOLOGIES

Sep 24, 2018

EMKA announced major enhancements of its easyTEL+ digital implanted telemetry. No more antennas inside cages,...

Quantitative characterization of metaphase cellular events using 3D imaging cellular events 

Yokogawa

Sep 20, 2018

In conventional monolayer cell culture systems, cell nuclei show dynamic and cyclic changes in theirshape and height....

A carboxymethyl dextran-based polymeric conjugate as the antigen carrier for cancer immunotherapy

Spectral Instruments Imaging

Sep 18, 2018

A polymeric conjugate, composed of a carboxymethyl dextran (CMD) as the backbone and ovalbumin (OVA) as a model foreign...

Oct 15, 2018

Adenovirus type B detection using the xxpress qPCR thermal cycler

BJS Biotechnologies

Sep 17, 2018

The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...

Show all topics (10)

Analysing “tissue sociology” with StrataQuest software

Feb 23, 2016

Cell in tissue plays a defined role as being a member of a small cell subunit within a larger organic structure. The development and differentiation of a given cell is defined by the parent cell and the local cell neighbourhood and environment. Consequently, the analysis of cells in their local environment is of major importance. StrataQuest analysis software contains specific image processing tools for the characterization of the social situation of cells in tissue. Apart from distance measurements there are tools to identify relationships of specific cells by selecting all cells in a local neighbourhood or functional group as a separate population.

The depicted skin sample was stained using the Multi-epitope Ligand Cartography (MELC) method (Fig. A). The tissue sample is subjected to repeated cycles of fluorescent staining, imaging and photo bleaching. Each cycle can use another antibody for a different antigen. The result is a set of images showing the distribution of each antigen in a different layer for the same sample.

The job for StrataQuest in this skin sample was to detect all cells and Melan-A positive areas (Fig. B). Based on this location information, the increase or decrease of the 90 antigen intensities in the areas vicinity was quantified . On this behalf, the Melan-A positive areas were automatically detected and distance transformation masks were established around each of them (Fig.C). The epidermis was also automatically detected (Fig. D).

StrataQuest has the capability to perform these automatic detection, measurement and analysis steps for any functional group of cells in such samples.

Application
Product news

Brand profile

TissueGnostics

TissueGnostics develops systems for automated identification and functional characterization of single cells in tissue sections, cultured and smeared cells as well as TMA. This includes automated image acquisition and image processing for immunohistochemistry, multi-chanel immunofluorescence and hematology.

Related products

Tissue analysis system for immunohistochemistry and immunofluorescence. TissueFAXSi Plus draws together features of HistoFAXS and TissueFAXS systems.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey