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IncuCyte webinar: high throughput approach to developing T- Cell immunotherapies

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Jun 17, 2019

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This event offers an overview of how live-cell analysis enables real-time analysis for the full workflow of the cells....

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Join the AVANCE Track & Keep your MRI on Track

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Your research depends on the scientific advances that you make with your MRI instrument. Take your instrument and your...

A novel method for isolating high-quality UHMW DNA from animal and human tissues

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From incubation to publication with IncuCyte S3 live-cell imaging system

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Over 2000 peer-reviewed publications worldwide and growing. Explore the IncuCyte publications library, an up-to-date...

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ParaVision 7 software

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The preclinical MRI software ParaVision 7 delivers unsurpassed image quality and enhanced productivity for both routine...

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Analysis of extracellular vesicles including exosomes by AMNIS imaging flow cytometer - June 8, 2016

May 23, 2016

Extracellular vesicles (EVs) such as exosomes (70 nm – 160 nm in diameter) and microvesicles (100 nm – 1,000 nm diameter) can be harvested from cell-culture supernatants and from all bodily fluids. Current standard techniques to visualize, quantify, and characterize EVs are electron microscopy, nanoparticle tracking analyses, and dynamic light scattering. To further characterize and discriminate EVs, more exact high-throughput technologies to analyze their surface are highly desired. Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm – 500 nm, a relatively new flow-cytometric method called “imaging flow cytometry” allows for the analysis of EVs smaller than 300 nm. This webinar on Wednesday, June 8, 2016 (6 pm CEST) will introduce viewers to the challenges, limitations, and pitfalls of flow cytometry-based EV analysis, and to the imaging flow cytometry methodology. Also covered will be techniques for analyzing exosomes, microvesicles, and apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.

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