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Scientists performed an unbiased analysis of functional diversity in colorectal cancer cells and their microenvironment using RNA-seq profiling of over 1,500 unsorted single cells from 11 primary tumors and matched normal mucosa (NM). To robustly interpret single-cell transcriptomes, we developed novel algorithms for normalizing expression estimates (pQ), clustering cells (RCA) and identifying differentially expressed genes (NODES). RCA identified 6 major cell types and multiple subtypes within colorectal samples.
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