Capturing rapid cellular events using simultaneous image acquisition

The Hermes WiScan imaging system provides a built-in solution for simultaneous imaging. It features image acquisition in four separate imaging channels, which can be operated in parallel or sequentially, according to the user’s requirements. This configuration allows for simultaneous measurements in live cell experiments on the one hand, and for extremely rapid scanning rates on the other hand. Successful acquisition of several fluorescence images with different wavelengths simultaneously depends on adequate experimental design, in which fluorophores are spectrally separated in order to avoid (or at least minimise) spectral bleed-through, which otherwise occur as a result of overlap between the emission spectra of probes. The Hermes WiScan® system offers a variety of optional fluorophore combinations for simultaneous acquisition to choose from, according to specific research
requirements.